Characterization and genome analysis of a novel Vibrio parahaemolyticus phage vB_VpP_DE17
Characterization and genome analysis of a novel Vibrio parahaemolyticus phage vB_VpP_DE17
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新型副溶血性弧菌噬菌体 vB_VpP_DE17 的表征和基因组分析
DOI：
10.1016/j.virusres.2021.198580
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发表时间：
2022-01-02
期刊：
影响因子：
5
通讯作者：
Tan, Zhiyuan
中科院分区：
文献类型：
--
作者：
Yang, Meiyan;Chen, Hanfang;Tan, Zhiyuan
从广州黄沙水产市场下水道中分离到一株感染副溶血性弧菌的新噬菌体VBVPP DE17。透射电子显微镜显示DE17具有二十面体头部(直径47+/-2 nm)和短的非收缩尾部(17+/-2 nm)。DE17基因组为双链线形DNA，全长43,397bp，GC含量为49.23%。总共预测了49个可能的开放阅读框架(ORF)，可分为6个模块：DNA代谢、裂解、包装、结构、附加功能和假设蛋白质。分类分析表明，该噬菌体属于斑点病毒亚科、斑点病毒科、斑点状病毒属。DE17的短潜伏期为5min，突发量为80pfu/cell。其最适温度范围为4℃~50℃，最适pH范围为5~10℃，紫外线照射20min即可完全灭活。没有发现转移RNA(TRNA)、毒力相关基因或抗生素耐药性基因。细菌激发试验表明，DE17在6h内对副溶血性弧菌有一定的抑制作用。DE17的鉴定、基因组分析和体外抗菌试验将进一步加深对噬菌体生物学和多样性的了解。
A novel phage vB_VpP_DE17, which infects Vibrio parahaemolyticus, was isolated from the sewer of the Huangsha aquatic market in Guangzhou. Transmission electron microscopy indicated that DE17 had an icosahedral head (47 +/- 2 nm diameter) and a short, non-contractile tail (17 +/- 2 nm). The genome of DE17 was a double-stranded linear DNA with a length of 43,397 bp and GC content of 49.23%. In total, 49 putative open reading frames (ORFs) were predicted and could be divided into six modules: DNA metabolism, lysis, packaging, structure, additional function, and hypothetical proteins. Taxonomic analysis revealed that the phage belonging to the genus of Maculvirus, Autographivirinae subfamily, Podoviridae family. DE17 had a short latent period of 5 min with burst size of 80 pfu/cell. Its optimum temperature and pH ranges were 4 degrees C-50 degrees C and 5-10, respectively; it was completely inactivated after 20 min of ultraviolet irradiation. No transfer RNA (tRNA), virulence associated, or antibiotic resistance genes were identified. Bacterial challenge test revealed that DE17 had a certain inhibitory effect on V. parahaemolyticus within 6 h. Characterization, genomic analysis and in vitro antibacterial assays of DE17 will further enhance our understanding of phage biology and diversity.