Kap promoter analysis in vivo:: a regulatory role for a truncated L1 repeat
Kap promoter analysis in vivo:: a regulatory role for a truncated L1 repeat
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DOI：
10.1016/s0303-7207(01)00538-x
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发表时间：
2001-07-05
影响因子：
4.1
通讯作者：
Catterall, JF
中科院分区：
文献类型：
--
作者：
Hardy, DO;Niu, EM;Catterall, JF
由 Kap 启动子的 1542 碱基对 (bp) 片段指导的报告基因表达对肾脏近端小管具有特异性，并受雄激素调节。在本研究中，在体内检查了 1542 bp 启动子的雄激素反应特征。还检查了肾脏和子宫中的雌激素反应。在由截短的启动子构建体产生的转基因小鼠品系中测定报告基因表达，其中存在于1542-bp远端部分的LI重复已被删除。报告基因的雄激素反应模式与内源性 Kap 相似。 1542 bp 启动子中的报告基因表达不会对肾脏中的雌激素做出反应，而围产期在子宫中的表达却会发生。 LI 的截断会导致报告基因表达丧失。我们得出结论，Kap 启动子附近存在的 LI 序列具有调节功能。 (C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
Reporter gene expression directed by a 1542-base pair (bp) fragment of the Kap promoter is specific to the proximal tubules of the kidney and androgen-regulated, In the present study, the characteristics of the androgen response from the 1542-bp promoter were examined in vivo. The estrogen response in the kidney and uterus was also examined. The reporter gene expression was assayed in lines of transgenic mice generated from a truncated promoter construct in which the LI repeat, present at the distal portion of the 1542-bp, had been deleted. The pattern of androgen response of the reporter gene is similar to that of the endogenous Kap. Reporter gene expression in the 1542-bp promoter does not respond to estrogen in the kidney, while perinatal expression in the uterus does occur. Truncation of the LI results in loss of reporter gene expression. We conclude that LI sequences present near the Kap promoter have a regulatory function. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.