CD34-/CD133+/VEGFR-2+ endothelial progenitor cell subpopulation with potent vasoregenerative capacities
CD34-/CD133+/VEGFR-2+ endothelial progenitor cell subpopulation with potent vasoregenerative capacities
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DOI：
10.1161/01.res.0000205765.28940.93
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发表时间：
2006-02-17
影响因子：
20.1
通讯作者：
Werner, N
中科院分区：
文献类型：
--
作者：
Friedrich, EB;Walenta, K;Werner, N
我们的目标是确定内皮祖细胞（EPC）的异质组内的功能重要的亚群。CD 133（+）祖细胞的活化细胞分选仪分析显示存在CD 34（+）和CD 34（-）亚群。CD 34（-）/133（+）祖细胞分化为CD 34（+）/133（+）EPC，对SDF-1的反应比这些祖细胞更强有力地粘附，并迅速回到人类志愿者肢体缺血的部位。在人冠状动脉粥样硬化斑块切除术样本中，稳定斑块中的CD 34（-）/133（+）比CD 34（+）/133（+）EPC少，而不稳定病变中的细胞数量随着比值的逆转而增加。在注射CD 34（-）/133（+）EPC的裸鼠中，与注射CD 34（+）/133（+）的小鼠相比，更多的共表达内皮标志物的移植细胞归巢到颈动脉病变内皮。在前者中，病变较小，再内皮化高于后者。我们鉴定了一个新的CD 34（-）/133（+）EPC亚群，它显然是“经典”CD 34（+）/133（+）EPC的前体，并且在归巢和血管修复方面比这些EPC功能更强大。
Our goal was to identify functionally important subpopulations within the heterogenous group of endothelial progenitor cells (EPC). Fluorescence-activated cell sorter analysis of CD133(+) progenitor cells revealed the presence of CD34(+) and CD34(-) subpopulations. CD34(-)/133(+) progenitors differentiate into CD34(+)/133(+) EPC, adhere more potently than these in response to SDF-1, and rapidly home to sites of limb ischemia in human volunteers. In human coronary atherectomy samples, fewer CD34(-)/133(+) than CD34(+)/133(+) EPC are present in stable plaques, whereas cell numbers increase with a reversion of the ratio in unstable lesions. In CD34(-)/133(+) EPC-injected nude mice, more transplanted cells coexpressing endothelial markers home to carotid artery lesion endothelium than in CD34(+)/133(+)-injected mice. In the former, lesions were smaller and reendothelialization higher than in the latter. We identified a new CD34(-)/133(+) EPC subpopulation, which is apparently a precursor of "classical" CD34(+)/133(+) EPC, and functionally more potent than these with respect to homing and vascular repair.