Cloning and characterization of a novel gene that encodes (S)-β-bisabolene synthase from ginger, Zingiber officinale
Cloning and characterization of a novel gene that encodes (S)-β-bisabolene synthase from ginger, Zingiber officinale
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DOI：
10.1007/s00425-010-1137-6
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发表时间：
2010-06-01
期刊：
影响因子：
4.3
通讯作者：
Misawa, Norihiko
中科院分区：
文献类型：
--
作者：
Fujisawa, Masaki;Harada, Hisashi;Misawa, Norihiko
生姜（学名：Ginger）含有一种主要由倍半萜和单萜组成的芳香油。我们从姜的幼嫩根茎中分离到一个编码倍半萜合酶的cDNA。officinale Roscoe、日本栽培品种“Kintoki”。命名为ZoTps 1的cDNA可能编码包含550个氨基酸残基的蛋白质，并且与已经从该属中分离的倍半萜烯脱氢酶具有49-53%的同一性。其中ZoTps 1与d-甲羟戊酸利用基因共表达沿着的重组大肠杆菌细胞导致仅用d-甲羟戊酸内酯补充物生产倍半萜（S）-β-红没药烯。这一结果表明ZoTps 1是生姜中的（S）-β-红没药烯合酶基因。ZoTPSl被认为催化（S）-β-红没药烯的形成，其中法呢基二磷酸转化为橙花苷基二磷酸，然后在1位和6位碳之间环化。ZoTps 1基因在生姜“Kintoki”的幼嫩根茎中检测到，而在叶片、根和成熟根茎中未检测到。
Ginger, Zingiber officinale Roscoe, contains a fragrant oil mainly composed of sesquiterpenes and monoterpenes. We isolated a cDNA that codes for a sesquiterpene synthase from young rhizomes of ginger, Z. officinale Roscoe, Japanese cultivar "Kintoki". The cDNA, designated ZoTps1, potentially encoded a protein that comprised 550 amino acid residues and exhibited 49-53% identity with those of the sesquiterpene synthases already isolated from the genus Zingiber. Recombinant Escherichia coli cells, in which ZoTps1 was coexpressed along with genes for d-mevalonate utilization, resulted in the production of a sesquiterpene (S)-beta-bisabolene exclusively with a d-mevalonolactone supplement. This result indicated that ZoTps1 was the (S)-beta-bisabolene synthase gene in ginger. ZoTPS1 was suggested to catalyze (S)-beta-bisabolene formation with the conversion of farnesyl diphosphate to nerolidyl diphosphate followed by the cyclization between position 1 and 6 carbons. The ZoTps1 transcript was detected in young rhizomes, but not in leaves, roots and mature rhizomes of the ginger "Kintoki".