Dynamics of gametocytes among Plasmodium falciparum clones in natural infections in an area of highly seasonal transmission
Dynamics of gametocytes among Plasmodium falciparum clones in natural infections in an area of highly seasonal transmission
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DOI：
10.1086/340638
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发表时间：
2002-06-15
影响因子：
6.4
通讯作者：
Babiker, HA
中科院分区：
文献类型：
--
作者：
Abdel-Wahab, A;Abdel-Muhsin, AMA;Babiker, HA
在苏丹东部一个季节性疟疾高度传播地区的居民中研究了恶性疟原虫克隆配子体产生的动态。逆转录聚合酶链反应被用来检测2个基因的表达，编码配子体特异性蛋白质，pfs25和pfg377，在寄生虫采样的个人在整个一年。一些在潮湿季节获得感染的患者被发现在接下来的干燥季节在明显没有蚊子传播的情况下存在亚专利配子体血症。多克隆感染中寄生虫的基因分型显示单个克隆的配子体生产有相当大的波动。旱季结束时出现的配子母细胞是该地区雨季后遗传复杂的周期性疟疾爆发的最可能来源。
The dynamics of gametocyte production in Plasmodium falciparum clones were studied in inhabitants of an area of highly seasonal malaria transmission in eastern Sudan. Reverse-transcriptase polymerase chain reaction was used to detect expression of 2 genes that encode gametocyte-specific proteins, pfs25 and pfg377, in parasites sampled from individuals throughout one year. Some patients who acquired infections during the wet season were found to harbor sub-patent gametocytemia through the following dry season in the apparent absence of mosquito transmission. Genotyping of parasites in multiclonal infections showed considerable fluctuation of gametocyte production by individual clones. The gametocytes present at the end of the dry season provide the most probable source of the genetically complex cyclical malaria outbreaks following the rainy season in this region.