L-Arginine and tetrahydrobiopterin protects against ischemia/reperfusion-induced endothelial dysfunction in patients with type 2 diabetes mellitus and coronary artery disease
L-Arginine and tetrahydrobiopterin protects against ischemia/reperfusion-induced endothelial dysfunction in patients with type 2 diabetes mellitus and coronary artery disease
复制标题
DOI：
10.1016/j.atherosclerosis.2008.08.034
复制
发表时间：
2009-05-01
期刊：
影响因子：
5.3
通讯作者：
Pernow, J.
中科院分区：
文献类型：
--
作者：
Settergren, M.;Bohm, F.;Pernow, J.
四氢生物蝶呤（BH 4）缺乏导致L-精氨酸和内皮型一氧化氮合酶（eNOS）解偶联水平降低可能导致内皮功能障碍。我们研究了L-精氨酸和BH 4给药对2型糖尿病和冠状动脉疾病（CAD）患者缺血再灌注（I/R）诱导的内皮功能障碍的影响。采用静脉闭塞法测定12例2型糖尿病、糖耐量减低和冠心病患者前臂血流，缺血20 min，再灌注60 min。在两次单独的研究中，从缺血15分钟开始，患者接受15分钟的L-精氨酸（20 mg/min）和BH 4（500 μ g/min）或0.9%盐水的臂内输注。与缺血前相比，再灌注15和30 min时，灌注生理盐水后，乙酰胆碱诱导的内皮依赖性血管舒张（EDV）明显减少（P
Diminished levels of L-arginine and endothelial nitric oxide synthase (eNOS) uncoupling through deficiency of tetrahydrobiopterin (BH4) May Contribute to endothelial dysfunction. We investigated the effect of L-arginine and BH4 administration on ischemia-reperfusion (I/R)-induced endothelial dysfunction in patients with type 2 diabetes and Coronary artery disease (CAD). Forearm blood flow was measured by venous occlusion plethysmography in 12 patients with type 2 diabetes or impaired glucose tolerance and CAD.Forearm ischemia was induced for 20 min, followed by 60 min of reperfusion. The patients received a 15 min intra-brachial infusion Of L-arginine (20 mg/min) and BH4 (500 mu g/min) or 0.9% saline starting at 15 min of ischemia on two separate study occasions. Compared with pre-ischemia the endothelium-dependent vasodilatation (EDV) induced by acetylcholine was significantly reduced at 15 and 30 min of reperfusion when saline was infused (P