Triple helix formation with the promoter of human α1(I) procollagen gene by an antiparallel triplex-forming oligodeoxyribonucleotide
Triple helix formation with the promoter of human α1(I) procollagen gene by an antiparallel triplex-forming oligodeoxyribonucleotide
复制标题
DOI：
10.1093/nar/26.22.5218
复制
发表时间：
1998-11-15
影响因子：
14.9
通讯作者：
Guntaka, RV
中科院分区：
文献类型：
--
作者：
Nakanishi, M;Weber, KT;Guntaka, RV
脊椎动物的α 1（I）前胶原基因的启动子含有两段连续的多嘧啶/多嘌呤序列，称为C1（-140至-170）和C2（-171至-200）。反平行三聚体形成上游寡核苷酸与C1形成有效的三链体。人的C1区与啮齿类动物的α 1（I）启动子有7个核苷酸的差异，主要是A->G转换。人三聚体形成寡脱氧核糖核苷酸（TFO）有效地形成稳定的三链体，其Kd类似于10-20 nM，而啮齿动物TFO的Kd类似于100 nM。突变分析表明，3或4个nt（-153至-155）对于这种更高的亲和力是足够的。TFOs特异性的人C1抑制转录从人类启动子在体外的Hela细胞核提取物和在体内培养的鸡胚成纤维细胞。
The promoters of alpha 1(I) procollagen genes of vertebrates contain two contiguous stretches of polypyrimidine/polypurine sequences, referred to as C1 (-140 to -170) and C2 (-171 to -200). Antiparallel tripler-forming upstream oligonucleotides form efficient triplexes with C1. The C1 tract of human differs from rodent alpha 1(I) promoters by 7 nt which are mainly A-->G transitions. Human tripler-forming oligodeoxyribonucleotide (TFO) formed stable triplexes efficiently with a K-d of similar to 10-20 nM compared with a K-d Of similar to 100 nM for rodent TFO. Mutational analysis indicated that 3 or 4 nt (-153 to -155) are sufficient for this higher affinity. TFOs specific for human C1 inhibited transcription from human promoter both in vitro in Hela cell nuclear extracts and in vivo in cultured chick embryo fibroblasts.