Circular RNAs Co-Precipitate with Extracellular Vesicles: A Possible Mechanism for circRNA Clearance.
Circular RNAs Co-Precipitate with Extracellular Vesicles: A Possible Mechanism for circRNA Clearance.
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DOI：
10.1371/journal.pone.0148407
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Parker R
中科院分区：
文献类型：
--
作者：
Lasda E;Parker R
后剪接环状rna （circRNAs）普遍存在于许多真核生物系统中，由数千种不同的基因剪接而成。在研究中，circrna通常是高度稳定的，细胞降解和/或从细胞中清除circrna的机制是未知的。在这里，我们研究了细胞将环状rna清除到细胞外空间的可能性，可能是在释放的囊泡（如外泌体和微囊泡）内。从三种不同的细胞系和检测多种环状rna中，我们发现从细胞培养条件培养基中回收的细胞外囊泡（ev）制剂含有已建立的环状rna。此外，与产生细胞相比，这些环状rna在EV制剂中比其线性对应物富集。这支持了这样一种观点，即通过ev释放从细胞排出到细胞外空间可能是细胞清除环状rna的一种机制。此外，由于ev可以被其他细胞吸收，因此分泌的环状rna可能有助于细胞间的通信。
Backspliced circular RNAs (circRNAs) are prevalent in many eukaryotic systems and are spliced from thousands of different genes. Where examined, circRNAs are often highly stable and the mechanisms by which cells degrade and/or clear circRNAs from the cells are unknown. Here we investigated the possibility that cells can eliminate circRNAs into extracellular space, possibly within released vesicles such as exosomes and microvesicles. From three different cell lines and examining multiple circRNAs, we show that extracellular vesicle (EVs) preparations recovered from cell culture conditioned media contain established circRNAs. Moreover, these circRNAs are enriched over their linear counterparts within EV preparations when compared to the producing cells. This supports the idea that expulsion from cells into extracellular space, as by EVs release, can be a mechanism by which cells clear circRNAs. Moreover, since EVs can be taken up by other cells, excreted circRNAs could contribute to cell to cell communication.