The kinetics of cultured human glioma cells
The kinetics of cultured human glioma cells
复制标题
培养的人神经胶质瘤细胞的动力学
DOI：
--
复制
发表时间：
1975
影响因子：
12.7
通讯作者：
Charles B. Wilson
中科院分区：
文献类型：
--
作者：
T. Hoshino;M. Barker;Charles B. Wilson
研究了脑肿瘤单层细胞培养的动力学，这些细胞来源于脑肿瘤，在手术切除前用~3H-胸腺嘧啶核苷标记。放射自显影分析表明：1.原代培养的细胞类型分布与体内亲本肿瘤的存活区域相似；2.培养的第一周构成滞后期；3.在培养的第二周开始旺盛的增殖。~(14)C-胸腺嘧啶核苷双标记表明，原代培养细胞在第一周的脉搏标记指数(PLI)*低于亲代肿瘤细胞，而在第二周培养细胞的PLI增加。相反，我们发现我们实验室长期建立的培养物在传代后的头几天有一个很高的PLI，迅速达到最大值，然后在传代后一周急剧下降。PLI的后一种变化对应于以下生长模式：1.指数增长；2.由于中等强度的耗尽而处于静止阶段；或3.由于过度拥挤而处于平台期。有迹象表明，一些在亲本肿瘤中具有分裂能力的细胞类型在转移到组织培养后失去了这种能力。
SummaryThe kinetics of monolayer cell cultures, derived from brain tumors which had been labeled with3H-thymidine just prior to surgical removal, have been investigated. Analysis of autoradiographs indicates that: 1. the distribution of cell types in primary cultures is similar to that of the viable areas of the parent tumorin vivo; 2. the first week of culture constitutes a lag phase; and 3. vigorous proliferation commences during the second week of culture. Double labeling of the tritiated cultures with14C-thymidine indicates that the pulse labeling index (PLI)* of primary cultures during the first week is lower than that of the parent tumor and that the PLI of the culture increases during the second week. In contrast, we found that long-term established cultures in our laboratory have a high PLI during the first few days after passage, quickly reach a maximum, and then drop precipitously 1 week after passage. These latter variations in PLI correspond to the following growth pattern: 1. exponential growth; 2. a stationary phase due to medium exhaustion; or 3. a plateau due to overcrowding. There are indications that some cell types, which have the capacity to divide in the parent tumor, lose this ability after transfer to tissue culture.