2‐D reference map of Bacillus anthracis vaccine strain A16R proteins
2‐D reference map of Bacillus anthracis vaccine strain A16R proteins
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DOI：
10.1002/pmic.200401322
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发表时间：
2005-11
期刊：
影响因子：
3.4
通讯作者：
Junjun Wang;Tianyi Ying;Hengliang Wang;Zhaoxing Shi;Mingzhu Li;Kun He;E. Feng;Jie-Siou Wang
中科院分区：
文献类型：
--
作者：
Junjun Wang;Tianyi Ying;Hengliang Wang;Zhaoxing Shi;Mingzhu Li;Kun He;E. Feng;Jie-Siou Wang
炭疽杆菌是一种重要的病原菌，它能引起致命的吸入性炭疽病，并可能被用作生物武器或生物恐怖分子。在这项研究中，2-DE参考图和数据库的B。anthracis A16 R的构建。总共处理了534个点，鉴定了代表299种蛋白质的406个点。凝胶估计的pI和分子量大多与其理论预测吻合良好，但也存在一些差异。斑点和蛋白质对应分析表明，翻译后修饰可能是常见的B。炭疽病通过MASCOT搜索，得到了B. anthracis，B. B. cereus和B.通过蛋白质水平和B中可能存在的注释错误进一步验证了苏云金杆菌。炭疽菌艾姆斯0581菌株基因组中的蛋白质，能量代谢、脂肪酸和磷脂代谢、蛋白质合成和细胞过程中的蛋白质占很大一部分。同时，对27个假设蛋白质进行了实验验证。在最近的孢子相关研究中，有28种蛋白质也被鉴定为孢子组分，这表明它们可能在生长、孢子形成和生长的不同阶段发挥作用。关于所有鉴定的蛋白质的图谱和信息可在互联网上获得，网址为http：//www.mpiib-berlin.mpg.de/2D-PAGE和http://www.proteomics.com.cn。
Bacillus anthracis has always been an important pathogen because it can cause lethal inhalational anthrax, and may be used as a bioweapon or by bioterrorists. In this study, a 2‐DE reference map and database of B. anthracis A16R was constructed. In total, 534 spots were processed, and 406 spots representing 299 proteins were identified. Gel‐estimated pIs and molecular masses mostly matched well with their theoretical predictions, but some discrepancies also existed. Spot and protein corresponding analysis revealed that post‐translational modifications might be common in B. anthracis. Through the MASCOT search, the similarity of B. anthracis, B. cereus and B. thuringiensis was further verified by protein level and a possible annotation error in B. anthracis strain Ames 0581 genome was found. Proteins of energy metabolism, fatty acid and phospholipid metabolism, protein synthesis, and cellular processes represented a large part of the most abundant proteins. At the same time, 27 hypothetical proteins were experimentally proved. There were 28 proteins also identified as spore composition in recently spore‐related research, which indicated that they might play some roles in different phases such as growth, sporulation and outgrowth. Maps and information about all identified proteins are available on the Internet at http://www.mpiib‐berlin.mpg.de/2D‐PAGE and http://www.proteomics.com.cn.