Structural identification of uric acid and its monohydrates by IR-UV double resonance spectroscopy
Structural identification of uric acid and its monohydrates by IR-UV double resonance spectroscopy
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红外-紫外双共振光谱法鉴定尿酸及其一水合物的结构
DOI：
10.1039/c1cp22540e
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发表时间：
2011
影响因子：
3.3
通讯作者：
H.Saigusa
中科院分区：
文献类型：
--
作者：
H.Asami;S.Urashima;H.Saigusa
采用红外-紫外双共振光谱法对激光解吸和超音速射流冷却技术制备的尿酸及其一水合团簇的互变异构体和同分异构体结构进行了鉴定。获得裸尿酸的红外光谱显示了四个不同的NH拉伸跃迁，可分配给最稳定的三酮形式。我们还观察到两个最稳定的单水合团簇，每一个都以三键形式存在尿酸，并且水与N3H或N9H位点结合。结果表明，用红外纯化光谱法可以分离出这些一水合物的R2PI光谱。
We have employed IR-UV double resonance spectroscopy to identify the tautomeric and isomeric structures of uric acid and its monohydrated clusters which are produced by the techniques of laser-desorption and supersonic-jet cooling. The IR spectrum obtained for bare uric acid exhibits four distinct NH stretching transitions assignable to those of the most stable triketo form. We have also observed the two most stable monohydrated clusters, each with uric acid in the triketo form and water bonded to either the N3H or N9H site. It is demonstrated that the R2PI spectra of these monohydrates can be separated by using the IR-purified spectroscopic method.