Frog skeletal muscle thick filaments are three-stranded.
Frog skeletal muscle thick filaments are three-stranded.
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青蛙骨骼肌肉厚细丝是三链。
DOI：
10.1083/jcb.96.6.1797
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发表时间：
1983-06
影响因子：
7.8
通讯作者：
Stewart, M
中科院分区：
文献类型：
--
作者：
Kensler, R W;Stewart, M
已经开发出一种用于分离和负染色脊椎动物骨骼肌粗丝的程序，以保留肌球蛋白横桥的排列。这些细丝的电子显微照片显示出与轴向重复为 42.9 nm 的交叉桥相关的明显周期性。这些图像的光学衍射图显示出清晰的层线，并且在质量上与已发表的 X 射线衍射图相似，只是 1/14.3-nm 经向反射稍弱。对这些细丝的负染色图像进行计算机图像分析，可以明确确定股线的数量。从层线数据重建的图像和第一层线内部最大值的相位分析都仅与三链结构一致，并且不能与二链或四链模型相一致。
A procedure has been developed for isolating and negatively staining vertebrate skeletal muscle thick filaments that preserves the arrangement of the myosin crossbridges. Electron micrographs of these filaments showed a clear periodicity associated with crossbridges with an axial repeat of 42.9 nm. Optical diffraction patterns of these images showed clear layer lines and were qualitatively similar to published x-ray diffraction patterns, except that the 1/14.3-nm meridional reflection was somewhat weaker. Computer image analysis of negatively stained images of these filaments has enabled the number of strands to be established unequivocally. Both reconstructed images from layer line data and analysis of the phases of the inner maxima of the first layer line are consistent only with a three-stranded structure and cannot be reconciled with either two- or four-stranded models.