Analysis and suppression of DNA polymerase pauses associated with a trinucleotide consensus
Analysis and suppression of DNA polymerase pauses associated with a trinucleotide consensus
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DOI：
10.1093/nar/24.14.2774
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发表时间：
1996-07-15
影响因子：
14.9
通讯作者：
Chamberlin, MJ
中科院分区：
文献类型：
--
作者：
Mytelka, DS;Chamberlin, MJ
我们已经研究了一类新的DNA序列，导致DNA聚合酶暂停。这些序列具有中心共有Py-G-C，并且不一定与DNA模板中的Its发夹相邻。由于大多数共有序列在标准条件下不会引起暂停，因此必须出现额外的模板特征，使得难以在这些位置掺入核苷酸。我们认为，这些停顿是由于限制，使涉及核苷酸选择的构象变化更加困难。这些停顿会模糊DNA测序梯的部分，并阻止聚合酶链反应的DNA扩增。添加甜菜碱和一些相关化合物可以缓解这些停顿。
We have studied a novel class of DNA sequences that cause DNA polymerases to pause. These sequences have the central consensus Py-G-C and are not necessarily adjacent Its hairpins in the DNA template. Since most consensus sequences do not cause pauses under standard conditions, additional template features must arise that make it difficult to incorporate nucleotides at these positions. We believe that these pauses result from constraints that make the conformation change involved in nucleotide selection more difficult. These pauses can obscure parts ok DNA sequencing ladders and prevent DNA amplification by the polymerase chain reaction. The addition of betaine, and some related compounds, relieves these pauses.