Potential-step coulometry of D-glucose using a novel FAD-dependent glucose dehydrogenase
Potential-step coulometry of D-glucose using a novel FAD-dependent glucose dehydrogenase
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DOI：
10.1007/s00216-006-0421-6
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发表时间：
2006-10-01
影响因子：
4.3
通讯作者：
Kano, Kenji
中科院分区：
文献类型：
--
作者：
Tsujimura, Seiya;Kojima, Shinki;Kano, Kenji
本文描述了使用新型 FAD 依赖性葡萄糖脱氢酶检测 D-葡萄糖的间歇式库仑系统的构建和表征。为了克服抗坏血酸和尿酸盐等干扰物的问题，提出了一种电位阶跃法，通过选择具有适当形式电位的介体来分离干扰物和D-葡萄糖的电解反应。干扰物的快速氧化消耗在第一步中进行，而介质和葡萄糖仍然减少。第二步，介体立即被氧化，随后在醛糖1-差向异构酶的帮助下发生D-葡萄糖的生物电催化氧化。在这项研究中，选择形式电位为0.6 V的八氰钼酸钾（IV）相对于Ag垂直棒AgCl作为介体，考虑到非均相电子转移动力学和电位窗口，第一和第二电解电位分别设置为0.4 V和0.8 V。就 D-葡萄糖量而言，充电时的背景校正响应相当于 99 +/- 2% 的效率。
This paper describes the construction and characterization of a batch-type coulometric system for the detection of D-glucose using a novel FAD-dependent glucose dehydrogenase. In order to overcome the problem of interferents, such as ascorbate and urate, a potential-step method was proposed to separate the electrolysis reactions of interferents and D-glucose by selecting a mediator possessing an appropriate formal potential. The rapid oxidative consumption of the interferents proceeded in the first step, whereas the mediator and glucose remained reduced. In the second step, the mediator was immediately oxidized, and subsequent bioelectrocatalytic oxidation of D-glucose occurred with the aid of aldose 1-epimerase. In this study, potassium octacyanomolybdate (IV) with a formal potential of 0.6 V vs. Ag vertical bar AgCl was chosen as a mediator, and the first and second electrolysis potentials were set at 0.4 Vand 0.8 V, respectively, by considering the heterogeneous electron-transfer kinetics and the potential window. The background-corrected response in charge corresponded to 99 +/- 2% efficiency in terms of the amount of D-glucose.