Growth hormone and epidermal growth factor together enhance amino acid transport systems B(0,+) and A in remnant small intestine after massive enterectomy.
Growth hormone and epidermal growth factor together enhance amino acid transport systems B(0,+) and A in remnant small intestine after massive enterectomy.
复制标题
生长激素和表皮生长因子共同增强大肠切除术后残余小肠中的氨基酸转运系统 B(0, ) 和 A。
DOI：
10.1016/s0022-4804(03)00185-9
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Sax,HarryC
中科院分区：
文献类型：
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作者：
Ray,EdwardC;Avissar,NellyE;Vukcevic,Dubravka;Toia,Liana;Ryan,CharlotteK;Berlanga-Acosta,Jorge;Sax,HarryC
大量肠切除术后2周，钠依赖性刷状缘营养转运减少。这种下调是改善了1周的胃肠外生长激素（GH）和表皮生长因子（EGF）开始1周后切除。我们假设，谷氨酰胺（GLN）的运输将增强较早和较长的生长因子输注，与Na+依赖的GLN运输系统A，B 0，+，和B 0/ASCT 2的差异影响。材料和方法新西兰白色兔进行70%小肠切除术，然后立即接受肠外EGF，GH，两者，或都没有2周。测定了空肠和回肠刷状缘膜囊对Na+依赖性~ 3 H-GLN的摄取，然后通过竞争性抑制测定了系统A、B 0、+和B 0的贡献。在未切除的动物中，空肠（A，9%; B 0，+，20%;和B 0，71%）和回肠（A，13%; B 0，+，27%;和B 0，60%）中系统的相对贡献相似。Na+依赖GLN摄取减少了一半切除，未经处理的控制，主要是因为降低B 0活动。EGF或GH单独使用不影响Na+依赖的GLN转运，但联合使用时，与切除对照组相比，残留回肠和空肠的摄取分别增加了144%和150%（P < 0.05）。这是延迟和较短持续时间联合治疗的两倍。这种增强是由于6.1- 8.2倍增加系统A以及3.8- 3.9倍增强系统B 0，+活性在残余回肠和空肠（P < 0.01）。
BACKGROUNDSodium-dependent brush border nutrient transport is decreased 2 weeks after massive enterectomy. This downregulation is ameliorated by a 1-week infusion of parenteral growth hormone (GH) and epidermal growth factor (EGF) started 1 week after resection. We hypothesized that glutamine (GLN) transport would be enhanced by earlier and longer growth factor infusion, with differential effects on the Na+-dependent GLN transport systems A, B0,+, and B0/ASCT2.MATERIALS AND METHODSNew Zealand White rabbits underwent 70% small bowel resection then immediately received parenteral EGF, GH, both, or neither for 2 weeks. Na+-dependent3H-GLN uptake by jejunal and ileal brush-border membrane vesicles was measured and the contribution of systems A, B0,+, and B0then determined by competitive inhibition. Data were analyzed using one-way analysis of variance.RESULTSIn nonresected animals, the relative contribution of the systems was similar in jejunum (A, 9%, B0,+, 20%; and B0, 71%) and ileum (A, 13%; B0,+, 27%; and B0, 60%). Na+-dependent GLN uptake was reduced by half in resected, untreated controls, primarily because of decreased B0activity. EGF or GH alone did not affect Na+-dependent GLN transport, but as a combination, increased uptake in the residual ileum and jejunum by 144% and 150%, respectively, over resected controls (P < 0.05). This was twice that achieved by delayed and shorter-duration combination treatment. This augmentation was due to a 6.1- to 8.2-fold increase in system A as well as a 3.8- to 3.9-fold enhancement of system B0,+activity in remnant ileum and jejunum (P < 0.01).CONCLUSIONSParenteral EGF and GH, given in combination for 2 weeks immediately after massive enterectomy, synergistically enhance GLN uptake by systems A and B0,+.