STRUCTURAL FEATURES AND RESTRICTED EXPRESSION OF A HUMAN ALPHA-TUBULIN GENE
STRUCTURAL FEATURES AND RESTRICTED EXPRESSION OF A HUMAN ALPHA-TUBULIN GENE
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DOI：
10.1093/nar/13.1.207
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
COWAN, NJ
中科院分区：
文献类型：
--
作者：
HALL, JL;COWAN, NJ
描述了人α-微管蛋白基因（Bα 1）的核苷酸序列。该基因与大鼠α-tubulln基因在其编码区、3′-非翻译区以及其最大内含子的片段中具有广泛的同源性。然而，除了三个短的保守同源区，大鼠和人类基因的5′侧翼区是不相关的。因此，这些编码相同蛋白质的基因在不同启动子的影响下转录。使用来自不同组织的各种转化细胞的RNA进行的印迹分析表明，人α-微管蛋白基因的表达仅限于神经源性细胞。在神经细胞类型中，Bα 1表达进一步限于形态分化的贴壁细胞。所提供的数据表明，Bαl基因编码一个突出的神经元和神经胶质α-微管蛋白，并且Bαl表达是这些细胞分化状态的函数。
The nucleotide sequence of a human α-tubulin gene (bαl) is described. This gene is extensively homologous to a rat α-tubulln gene in its coding regions, 3′-untranslated region and, indeed, in segments of its largest intron. However, with the exception of three short conserved blocks of homology, the 5′ flanking regions of the rat and human genes are unrelated. Hence, these genes each encoding an identical protein are transcribed under the influence of divergent promoters.Blot analyses using RNA from a variety of transformed cells derived from different tissues indicate that expression of the human α-tubulin gene is restricted to cells of neurological origin. Among neurological cell types bαl expression is further restricted to adherent cells that are morphologically differentiated. The data presented suggest that the bαl gene encodes a prominent neuronal and glial α-tubulin and that bαl expression is a function of the differentiated state of these cells.