Supplementation of cryopreservation medium with TAT-Peroxiredoxin 2 fusion protein improves human sperm quality and function
Supplementation of cryopreservation medium with TAT-Peroxiredoxin 2 fusion protein improves human sperm quality and function
复制标题
在冻存培养基中添加 TAT-Peroxiredoxin 2 融合蛋白可改善人类精子的质量和功能
DOI：
10.1016/j.fertnstert.2018.07.008
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发表时间：
2018-11-01
影响因子：
6.7
通讯作者：
Wang, Xiong
中科院分区：
文献类型：
--
作者：
Liu, Juan;Wang, Wenting;Wang, Xiong
目的：探讨在冷冻保存介质中添加 TAT-PRDX2 蛋白对解冻后精子质量和功能的潜在影响。设计：体外前瞻性研究。地点：医科大学附属医院。患者：50 名正常精子、50 名弱精子和 50 名少弱精子男性，因夫妇不孕而接受精液分析。干预措施：每个精液样本分为三份等分试样：新鲜、冷冻保存的对照（不含添加剂）和使用 TAT-PRDX2 蛋白冷冻保存。 主要结果指标：分析精子活力、活力、线粒体电位和 DNA 损伤以及活性氧 (ROS) 水平和脂质过氧化。通过顶体反应和无透明带仓鼠卵母细胞穿透试验来评估冻存精子的受精能力。结果：在正常精子组和弱精子组中，与各组冻存对照组相比，添加150 mg/mLTAT-PRDX2显着降低了细胞内ROS和丙二醛水平，增强了解冻后精子的活力和活力，但没有增加精子活力。对少弱精子群产生任何显着的保护作用。与冷冻保存对照组相比，仅在弱精子症组补充 TAT-PRDX2 后，线粒体潜能显着增加，而 DNA 碎片显着减少。虽然渗透率和渗透指数没有明显提高，但在正常精子组和弱精子组中，补充TAT-PRDX2明显减少了自发顶体反应，增加了钙离子载体诱导的顶体反应。结论：TAT-PRDX2蛋白通过降低细胞内ROS水平，有效地对精子发挥冷冻保护作用，从而改善解冻后的精子质量和功能，特别是对于弱精子样本。 TAT-PRDX2 蛋白是一种很有前景的添加剂，可用于开发新型高效精液冷冻保护剂。 (C) 2018 年，美国生殖医学会。
Objective: To investigate the potential effects of TAT-PRDX2 protein supplementation to the cryopreservation medium on post-thaw sperm quality and function.Design: In vitro prospective study.Setting: Medical university hospital.Patient(s): Fifty normozoospermic, 50 asthenozoospermic, and 50 oligoasthenozoospermic men undergoing semen analysis for couple infertility.Intervention(s): Each semen sample was divided into three aliquots: fresh, cryopreserved control (without additive), and cryopreserved with TAT-PRDX2 protein.Main Outcome Measure(s): Sperm motility, viability, mitochondrial potential, and DNA damage as well as reactive oxygen species (ROS) levels and lipid peroxidation were analyzed. Acrosome reaction and zona-free hamster oocyte penetration tests were performed to assess the fertilization ability of cryopreserved spermatozoa.Result(s): In normozoospermic and asthenozoospermic groups, the addition of 150 mg/mLTAT-PRDX2 significantly reduced intracellular ROS and malondialdehyde levels and enhanced post-thaw sperm motility and viability when compared with the cryopreserved control of the respective groups but did not produce any significant protective effect in the oligoasthenozoospermic group. Mitochondrial potential was significantly increased, whereas DNA fragmentation was significantly decreased, after TAT-PRDX2 supplementation only in the asthenozoospermic group when compared with the cryopreserved control. Although the penetration rate and the penetration index were not markedly improved, TAT-PRDX2 supplementation obviously reduced spontaneous acrosome reaction and increased calcium ionophore-induced acrosome reaction in the normozoospermic and asthenozoospermic groups.Conclusion(s): TAT-PRDX2 protein effectively exerted cryoprotective effects on spermatozoa by reducing intracellular ROS level and thereby improved post-thaw sperm quality and function, especially for asthenozoospermic samples. TAT-PRDX2 protein is a promising additive for developing a new and highly efficient semen cryoprotectant. (C) 2018 by American Society for Reproductive Medicine.