Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly.
Circularly permuted GTPase YqeH binds 30S ribosomal subunit: Implications for its role in ribosome assembly.
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DOI：
10.1016/j.bbrc.2009.06.078
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发表时间：
2009-09-04
影响因子：
3.1
通讯作者：
Prakash, Balaji
中科院分区：
文献类型：
--
作者：
Anand, Baskaran;Surana, Parag;Bhogaraju, Sagar;Pahari, Sushmita;Prakash, Balaji
关键词：
YqeH 是一种循环排列的 GTP 酶，在细菌和真核生物（包括人类）中保守。它被证明对于细菌中小核糖体 (30S) 亚基的组装至关重要。然而，YqeH 是否与 30S 核糖体相互作用以及它如何参与 30S 组装尚不清楚。在这里，通过共沉降实验，我们报告 YqeH 与 GTP 结合形式的 30S 核糖体共缔合。为了探究 YqeH 是否在 30S 组装中充当 RNA 伴侣，我们检测了链解离和退火活性。虽然 YqeH 不表现出这些活性，但它结合非特异性单链和双链 RNA，与 30S 结合不同，它独立于 GTP/GDP 结合，并且不影响内在的 GTP 水解速率。此外，S5（一种参与 30S 组装初始阶段的核糖体蛋白）被发现可促进 YqeH 的 GTP 水解和 RNA 结合活性。
YqeH, a circularly permuted GTPase, is conserved among bacteria and eukaryotes including humans. It was shown to be essential for the assembly of small ribosomal (30S) subunit in bacteria. However, whether YqeH interacts with 30S ribosome and how it may participate in 30S assembly are not known. Here, using co-sedimentation experiments, we report that YqeH co-associates with 30S ribosome in the GTP-bound form. In order to probe whether YqeH functions as RNA chaperone in 30S assembly, we assayed for strand dissociation and annealing activity. While YqeH does not exhibit these activities, it binds a non-specific single and double-stranded RNA, which unlike the 30S binding is independent of GTP/GDP binding and does not affect intrinsic GTP hydrolysis rates. Further, S5, a ribosomal protein which participates during the initial stages of 30S assembly, was found to promote GTP hydrolysis and RNA binding activities of YqeH.
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