MELTING, computing the melting temperature of nucleic acid duplex
MELTING, computing the melting temperature of nucleic acid duplex
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DOI：
10.1093/bioinformatics/17.12.1226
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发表时间：
2001-12-01
期刊：
影响因子：
5.8
通讯作者：
Le Novère, N
中科院分区：
文献类型：
--
作者：
Le Novère, N
熔化计算了寡核苷酸双链螺旋-螺旋转变的焓和熵，然后计算了它的熔化温度。该程序采用最近邻法。这组热力学参数可以很容易地定制。该程序提供了几种盐浓度的校正方法。熔化是一个免费的程序，免费提供，并且是开源的。提供了Perl脚本来展示如何使用熔化来构建更雄心勃勃的程序。
MELTING computes the enthalpy and entropy of an oligonucleotide duplex helix-coil transition, and then its melting temperature. The program uses the method of nearest-neighbours. The set of thermodynamic parameters can be easily customized. The program provides several correction methods for the concentration of salt. MELTING is a free program, available at no cost and open-source. Perl scripts are provided to show how MELTING can be used to construct more ambitious programs.