Real-time detection of serotonin release from enterochromaffin cells of the guinea-pig ileum
Real-time detection of serotonin release from enterochromaffin cells of the guinea-pig ileum
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DOI：
10.1111/j.1365-2982.2004.00572.x
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发表时间：
2004-10-01
影响因子：
3.5
通讯作者：
Bertrand, PP
中科院分区：
文献类型：
--
作者：
Bertrand, PP
5-羟色胺（5-HT）含有肠嗜铬细胞（EC）可以检测化学或机械刺激在肠腔和响应释放5-HT本研究的目的是使用实时电化学检测方法检测释放的5-HT从少量的EC细胞。在豚鼠回肠中，5-HT从未受刺激、未麻痹的肠的基础释放由不同幅度但具有相似动力学的单个释放事件（8.4 +/- 1.8事件，0.33 +/- 0.06 Hz）组成。用电极局部压迫粘膜诱发峰值5-HT释放为12.3 +/- 2.8 mumol L-1，持续释放为3.0 +/- 0.7 mumol L-1。短暂应用乙酰胆碱（ACh）或卡巴胆碱引起一个短暂的峰值（5.7 ± 1.3 μ mol L-1，发生在35 +/- 18 s，n = 9），然后是5-HT的周期性释放（9.7 +/- 2.2事件，0.40 +/- 0.13 Hz，n = 6）。这项研究表明，5-HT的释放迅速发生，作为少数细胞的个体事件，并可在局部达到非常高的浓度。
Serotonin (5-hydroxytryptamine; 5-HT) containing enterochromaffin (EC) cells may detect chemical or mechanical stimuli in the intestinal lumen and respond with release of 5-HT The aim of this study was to use real-time electrochemical detection methods to detect release of 5-HT from small numbers of EC cells. In guinea-pig ileum, basal release of 5-HT from the unstimulated, unparalyzed intestine was composed of individual release events (8.4 +/- 1.8 events, 0.33 +/- 0.06 Hz) of different amplitudes but with similar kinetics. Local compression of the mucosa with the electrode evoked peak 5-HT release of 12.3 +/- 2.8 mumol L-1 with a sustained release of 3.0 +/- 0.7 mumol L-1. Brief application of acetylcholine (ACh) or carbachol elicited a transient peak (5.7 1.3 mumol L-1 occurring at 35 +/- 18 s, n = 9) followed by cyclic release of 5-HT (9.7 +/- 2.2 events, 0.40 +/- 0.13 Hz, n = 6). This study shows that the release of 5-HT occurs rapidly as individual events from a small number of cells and can reach very high concentrations locally.