Increase of nuclear bodies in bovine luteal cells after treatment with human chorionic gonadotropin.
Increase of nuclear bodies in bovine luteal cells after treatment with human chorionic gonadotropin.
复制标题
用人绒毛膜促性腺激素处理后牛黄体细胞核体增加。
DOI：
10.1095/biolreprod38.2.453
复制
发表时间：
1988
影响因子：
3.6
通讯作者：
Rao,CV
中科院分区：
文献类型：
--
作者：
Chegini,N;Rao,CV
Nuclear bodies, which are dynamic components of nuclei indicating the level of nuclear activation and cellular transcriptional and translational events, were found in small numbers (2 to 4 nuclear profiles per 150 examined) in control luteal tissue. The number of nuclear bodies, however, greatly increased after incubation with increasing concentrations of human chorionic gonadotropin (hCG). Either one simple or one complex, but very rarely two or more nuclear bodies per nuclear profile, were found in response to hCG. The nuclear bodies increased by 1 h of incubation with hCG at 38°C (p < 0.01), which further increased by 2 h, remained elevated until 8 h (p < 0.01), and then returned to control levels by 12 h, Thirty to forty percent of the nuclear bodies that increased in response to hCG were complex nuclear bodies; these are larger than simple nuclear bodies, and contain a fibrillar capsule surrounding the electron-dense or granular core of tightly packed osmophilic material. While hCG increased the number of nuclear bodies similarly at 22°C and 38°C, it had no effect at 4°C. hCG had no effect on nuclear bodies in luteal fibroblasts and endothelial cells or in bovine liver or kidney cells, suggesting that the effect of hCG was cell- and tissue-specific. High concentrations of human prolactin (hPRL), human follicle-stimulating hormone (hFSH), or prostaglandin (PG) E1or PGF2αhad no effect on nuclear bodies in luteal cells, suggesting that the hCG effect was hormone-specific. Human luteinizing hormone (hLH) also increased the number of nuclear bodies in luteal cells, with three differences from that seen with hCG. First, hLH caused a smaller increase of nuclear bodies; second, longer periods of incubation with hLH were required for an increase in number of complex nuclear bodies; and third, even then, a fewer number of complex nuclear bodies appeared in response to hLH as compared to hCG. The addition of hCG with hLH resulted in appearance of nuclear bodies similar to the effects of treatment with hLH alone. Dibutyryl cyclic adenosine 3′,5′-monophosphate had no effect on number of nuclear bodies in bovine luteal cells, suggesting that the hCG and hLH increase of nuclear bodies could not have been mediated by this cyclic nucleotide.
