Uptake, excretion and toxicity of nano-sized latex particles on medaka (Oryzias latipes) embryos and larvae
Uptake, excretion and toxicity of nano-sized latex particles on medaka (Oryzias latipes) embryos and larvae
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DOI：
10.1016/j.aquatox.2011.08.020
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发表时间：
2011-10-01
影响因子：
4.5
通讯作者：
Kinoshita, Masato
中科院分区：
文献类型：
--
作者：
Manabe, Masahiko;Tatarazako, Norihisa;Kinoshita, Masato
纳米颗粒是指直径小于等于100 nm的颗粒，近年来随着这些颗粒应用的增加，其对人类和动物生理的潜在影响也在增加。然而，关于纳米颗粒对水生生物生理学的影响知之甚少。在这项研究中，我们研究了纳米尺寸的荧光乳胶颗粒对淡水鱼青鳉（Oryzias latipes）的影响。将青鳉暴露于四种不同类型的荧光乳胶颗粒，并检查了青鳉幼虫对荧光乳胶颗粒的吸收、排泄以及纳米颗粒积累对存活率的影响。这些是荧光乳胶颗粒，其直径为50和500 nm的非官能化的和直径为50和500 nm的羧基官能化的。与胚胎暴露于直径为500 nm的颗粒（颗粒500 nm和颗粒c500 nm）时相比，暴露于直径为50 nm的非官能化和羧基官能化颗粒（颗粒50 nm和颗粒c50 nm）的鱼胚胎中的荧光强度显著更高。此外，与较大颗粒（颗粒500和颗粒c500 nm）相比，纳米级颗粒（颗粒50和颗粒c50 nm）从胚胎中的排泄相当缓慢。此外，暴露于小杯中纳米尺寸颗粒的幼虫的存活率显著低于保持在较大容器中的鱼类的存活率。结果表明，虽然纳米荧光乳胶颗粒本身没有毒性，但与其他因素结合时会产生协同毒性效应，这对鱼类幼虫不利。(C)2011爱思唯尔有限公司版权所有。
Nanoparticles are particles with diameters of 100 nm or less. As the applications of these particles have increased in recent years, their potential impact on the physiology of humans and animals has also increased. However, little is known regarding the effect of nanoparticles on the physiology of aquatic organisms. In this study, we investigated the effect of nano-sized, fluorescent, latex particles on the freshwater fish, medaka (Oryzias latipes). Medakas were exposed to four different types of fluorescent latex particles and the uptake, excretion, and the effect of nanoparticle accumulation on survival rate in medaka larvae were examined. These are fluorescent latex particles, which are non-functionalized 50 and 500 nm in diameter and carboxyl-group functionalized 50 and 500 nm in diameter. Fluorescence intensity in fish embryos exposed to non-functionalized and carboxyl-group functionalized particles measuring 50 nm in diameter (Particle 50 nm and Particle c50 nm) was markedly higher compared to when embryos were exposed to particles measuring 500 nm in diameter (Particle 500 nm and Particle c500 nm). Moreover, the excretion of nano-sized particles (Particle 50 and Particle c50 nm) from embryos was considerably slow, compared to larger particles (Particle 500 and Particle c500 nm). In addition, the survival rate of larvae exposed to nano-sized particles in small cups was significantly lower than the survival rates of fish maintained in larger containers. The findings suggested that although the nano-sized fluorescent latex particles were not intrinsically toxic, a synergistic toxic effect arose in combination with other factors, which is not favorable for fish larvae. (C) 2011 Elsevier B.V. All rights reserved.