PRIMARY STRUCTURE AND GLYCOSYLATION OF THE S-LAYER PROTEIN OF HALOFERAX-VOLCANII
PRIMARY STRUCTURE AND GLYCOSYLATION OF THE S-LAYER PROTEIN OF HALOFERAX-VOLCANII
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DOI：
10.1128/jb.172.12.7111-7118.1990
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发表时间：
1990-12-01
影响因子：
3.2
通讯作者：
STROBEL, I
中科院分区：
文献类型：
--
作者：
SUMPER, M;BERG, E;STROBEL, I
火山古细菌 Haloferax volcanii（以前称为火山盐杆菌）的外表面覆盖有六边形堆积的表面 (S) 层。克隆并测序了编码 S 层蛋白的基因。成熟的多肽由 794 个氨基酸组成，前面是 34 个氨基酸残基的典型信号序列。 C 末端一段由 20 个氨基酸组成的高度疏水性片段可能充当跨膜结构域。苏氨酸残基簇位于该膜锚附近。 S层蛋白是含有N-和O-糖苷键的糖蛋白。葡萄糖基-(1→2)-半乳糖二糖与苏氨酸残基连接。对来自 Haloferax volcanii 和 Halobacter halobium 的 S 层糖蛋白的一级结构和糖基化模式进行了比较，发现它们表现出明显的差异，尽管电子显微照片的三维重建显示至少在迄今为止达到的 2.5 nm 水平上没有结构差异（M. Kessel、I. Wildhaber、S. Cohe 和 W. Baumeister、EMBO J. 7：1549-1554，1988）。
The outer surface of the archaebacterium Haloferax volcanii (formerly named Halobacterium volcanii) is covered with a hexagonally packed surface (S) layer. The gene coding for the S-layer protein was cloned and sequenced. The mature polypeptide is composed of 794 amino acids and is preceded by a typical signal sequence of 34 amino acid residues. A highly hydrophobic stretch of 20 amino acids at the C-terminal end probably serves as a transmembrane domain. Clusters of threonine residues are located adjacent to this membrane anchor. The S-layer protein is a glycoprotein containing both N- and O-glycosidic bonds. Glucosyl-(1 .fwdarw. 2)-galactose disaccharides are linked to threonine residues. The primary structure and the glycosylation pattern of the S-layer glycoproteins from Haloferax volcanii and from Halobacterium halobium were compared and found to exhibit distinct differences, despite the fact that three-dimensional reconstructions from electron micrographs revealed no structural differences at least to the 2.5-nm level attained so far (M. Kessel, I. Wildhaber, S. Cohe, and W. Baumeister, EMBO J. 7:1549-1554, 1988).