Visualization of early APC/T cell interactions in the mouse lung following intranasal challenge
Visualization of early APC/T cell interactions in the mouse lung following intranasal challenge
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DOI：
10.4049/jimmunol.167.12.6756
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发表时间：
2001-12-15
影响因子：
4.4
通讯作者：
Chaplin, DD
中科院分区：
文献类型：
--
作者：
Byersdorfer, CA;Chaplin, DD
我们使用带有或不带有共价结合 OVA 的荧光乳胶珠来促进小鼠肺部和引流支气管周围淋巴结 (LN) 中 Ag 运输的研究。鼻内给药后 6 小时和最多 48 小时，观察到组织实质中呈细胞内簇状的珠子。对支气管肺泡灌洗液中的珠阳性 (bead(+)) 细胞进行流式细胞术分析，结果表明这些细胞大多数为 CD11c(+)、F4/80(+) 和 CD11b(-)。此外，荧光显微镜证实肺组织中珠(+)细胞的主要亚群也是CD11c(+)。在引流的支气管周围淋巴结中，早在吸入后 6 小时，囊下窦中就存在少量珠子。 12 小时及之后，珠子 (+) 细胞仅定位于 LN T 区。 OVA 缀合乳胶珠除了在体外刺激幼稚的 OVA 特异性 DO11.10 转基因 T 细胞快速增殖外，还可以在体内招募 OVA 特异性 T 细胞。在某些情况下，气道激发后 6 小时，在肺组织中发现珠（+）APC 和 CD4（+）Th1 细胞相邻定位。因此，珠（+）APC与Ag特异性CD4（+）T细胞的相互作用在外周气道中比在引流支气管周围LN中发生的这些相同的相互作用更早发生。最后，在过继转移后，体外分化的 Th1 细胞在 Ag 攻击前聚集在肺组织和气道的外周部位，因此处于影响气道后续免疫反应的理想位置。
We have used fluorescent latex beads, with or without covalently conjugated OVA, to facilitate study of Ag trafficking in the mouse lung and draining peribronchial lymph node (LN). At 6 h, and up to 48 h after intranasal administration, beads were observed as intracellular clusters in the tissue parenchyma. Flow cytometry of bead-positive (bead(+)) cells from the bronchoalveolar lavage demonstrated that a majority of these cells are CD11c(+), F4/80(+), and CD11b(-). Furthermore, fluorescent microscopy confirmed that a major subset of bead(+) cells in the lung tissue was also CD11c(+). In the draining peribronchial LNs, small numbers of beads were present in the subcapsular sinus as early as 6 h after inhalation. By 12 h and beyond, bead(+) cells had localized exclusively to the LN T zone. OVA-conjugated latex beads, in addition to stimulating brisk proliferation of naive, OVA-specific DO11.10 transgenic T cells in vitro, could also recruit OVA-specific T cells in vivo. In some cases, bead(+) APCs and CD4(+) Th1 cells were found adjacently localized in the lung tissue 6 h after airway challenge. Thus, interactions of bead(+) APCs with Ag-specific CD4(+) T cells occurred earlier in the peripheral airways than these same interactions occurred in the draining peribronchial LN. Lastly, after adoptive transfer, in vitro differentiated Th1 cells accumulated at peripheral sites in the lung tissue and airways before Ag challenge and therefore were ideally positioned to influence subsequent immune reactions of the airway.