Effects of Microcystis aeruginosa on the expression of nuclear receptor genes in Daphnia similoides sinensis.
Effects of Microcystis aeruginosa on the expression of nuclear receptor genes in Daphnia similoides sinensis.
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DOI：
10.1016/j.ecoenv.2017.12.033
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发表时间：
2018-04
影响因子：
6.8
通讯作者：
Xiaoxue Xu;Ya-Nan Zhang;Shuixiu Peng;Jianxun Wu;D. Deng;Zhongze Zhou
中科院分区：
文献类型：
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作者：
Xiaoxue Xu;Ya-Nan Zhang;Shuixiu Peng;Jianxun Wu;D. Deng;Zhongze Zhou
核受体（NR）基因是一个保守的超家族，参与生物体的代谢、生殖、发育、稳态和资源分配。微囊藻能抑制水蚤的生长和繁殖。然而，无论M。aerodonosacan对水蚤NR基因表达的影响尚不清楚。根据之前的相似蚤中国亚种转录组数据，本研究总共识别出18个NR。在含M.在铜绿假单胞菌中，NR 1亚家族（E75 a、E75 b、HR 3、HR 96、NHR-1、HR 97 a、HR 97 g和NHR 97）和NR 2亚家族（RXR、TLL、PNR和SVP）的基因表达分别下调59%和79%。在含M.结果表明，克隆1和克隆2中有78%的基因表达趋势相似，而克隆3中有42%的基因表达趋势相反，表明不同耐藻基因型个体的适应性和分子机制不同。
Nuclear receptor (NR) genes form a conserved superfamily, which is involved in organism metabolism, reproduction, development, homeostasis, and resource allocation. Microcystisaeruginosacan inhibit the growth and reproduction of Daphnia. However, whetherM. aeruginosacan affect the expression of Daphnia NR genes is unknown. In total, 18 NRs were identified in this study based on previousDaphnia similoides sinensistranscriptome data. In treatments containingM. aeruginosa, the gene expression of the NR1 subfamily (E75a, E75b, HR3, HR96, NHR-1, HR97a, HR97g, and NHR97) and the NR2 subfamily (RXR, TLL, PNR, and SVP) were down-regulated 59% and 79%, respectively. In treatments containingM. aeruginosa, although the expression of 78% of the genes showed a similar trend in clones 1 and 2, the expression of 42% of the genes in clone 3 showed the opposite trend compared to clones 1 and 2, suggesting that the adaptability and molecular mechanism differ in individuals with differentMicrocystistolerance genotypes.