Cell wall synthesis in cotton roots after infection with Fusarium oxysporum
Cell wall synthesis in cotton roots after infection with Fusarium oxysporum
复制标题
尖孢镰刀菌感染后棉花根部细胞壁的合成
作者：
E. Rodríguez;K. Mendgen
尖孢镰刀菌sp.vasinfectumpenetration菌丝侵染棉花（GossypiumbarbadenseL.）根我们的特点是壁修改诱导的真菌感染的原皮层使用抗体对胼胝质，阿拉伯半乳聚糖蛋白，木葡聚糖，果胶，多聚半乳糖醛酸和鼠李糖半乳糖醛酸聚糖我在高压冷冻，冷冻取代根组织。使用定量免疫金标记，我们比较了菌丝接触前后的细胞壁，胞质分裂过程中胞间连丝的细胞板，和真菌接触引起的壁附着。在已经存在的壁中，真菌接触仅引起微小的修饰，例如木葡聚糖表位的增加。壁附着物大多表现出与细胞板相似的表位，只是壁附着物的愈伤组织含量要高得多。本研究表明，尖孢镰刀菌菌丝诱导的细胞壁附着是细胞壁正常合成和大量胼胝质合成的结果。附着物并不能阻止真菌的生长。
Fusarium oxysporumf. sp.vasinfectumpenetration hyphae infect living cells in the meristematic zone of cotton (Gossypium barbadenseL.) roots. We characterized wall modifications induced by the fungus during infection of the protodermis using antibodies against callose, arabinogalactan-proteins, xyloglucan, pectin, polygalacturonic acid and rhamnogalacturonan I in high-pressure frozen, freeze-substituted root tissue. Using quantitative immunogold labelling we compared the cell walls before and after hyphal contact, cell plates with plasmodesmata during cytokinesis, and wall appositions induced by fungal contact. In the already-existing wall, fungal contact induced only minor modifications such as an increase of xyloglucan epitopes. Wall appositions mostly exhibited epitopes similar to the cell plate except that wall appositions had a much higher callose content. This study shows that wall appositions induced byFusarium oxysporumhyphae are the result of normal cell wall synthesis and the addition of large amounts of callose. The appositions do not stop fungal growth.