FISH mapping of 57 BAC clones reveals strong conservation of synteny between Galliformes and Anseriformes
FISH mapping of 57 BAC clones reveals strong conservation of synteny between Galliformes and Anseriformes
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DOI：
10.1111/j.1365-2052.2007.01578.x
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发表时间：
2007-06-01
期刊：
影响因子：
2.4
通讯作者：
Vignal, A.
中科院分区：
文献类型：
--
作者：
Fillon, V.;Vignoles, M.;Vignal, A.
鸡和绿头鸭的核型具有典型的鸟类核型结构，包括少数大染色体对、大量难以区分的小染色体、Z性染色体和W性染色体。以前的显带研究表明，鸡和鸭之间有很大的相似性，但不可能对微染色体进行比较显带。为了在这两个物种之间建立精确的染色体对应，特别是在微染色体上，我们将以前分配给鸡基因组的57个BAC克隆杂交到鸭的中期传播。虽然大多数克隆显示出相似的定位，但我们在鸭子中发现了一些大染色体内的染色体内重排和额外的一对微染色体。将鸡微染色体特异的BAC克隆定位于分离鸭微染色体，并将定位到同一鸡微染色体的克隆杂交到同一鸭微染色体上，表现出高度的同时性保守性。这些结果表明，鸟类核型的进化是融合和/或分裂过程的结果，而不是易位的结果。
Karyotypes of chicken (Gallus gallus domesticus; 2n = 78) and mallard duck (Anas platyrhynchos; 2n = 80) share the typical organization of avian karyotypes including a few macrochromosome pairs, numerous indistinguishable microchromosomes, and Z and W sex chromosomes. Previous banding studies revealed great similarities between chickens and ducks, but it was not possible to use comparative banding for the microchromosomes. In order to establish precise chromosome correspondences between these two species, particularly for microchromosomes, we hybridized 57 BAC clones previously assigned to the chicken genome to duck metaphase spreads. Although most of the clones showed similar localizations, we found a few intrachromosomal rearrangements of the macrochromosomes and an additional microchromosome pair in ducks. BAC clones specific for chicken microchromosomes were localized to separate duck microchromosomes and clones mapping to the same chicken microchromosome hybridized to the same duck microchromosome, demonstrating a high conservation of synteny. These results demonstrate that the evolution of karyotypes in avian species is the result of fusion and/or fission processes and not translocations.