Nucleosome organization in the Drosophila genome
Nucleosome organization in the Drosophila genome
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DOI：
10.1038/nature06929
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发表时间：
2008-05-15
期刊：
影响因子：
64.8
通讯作者：
Pugh, B. Franklin
中科院分区：
文献类型：
--
作者：
Mavrich, Travis N.;Jiang, Cizhong;Pugh, B. Franklin
核小体位置的比较基因组学为理解染色质组织和转录机制如何共同进化提供了强有力的手段。在这里，我们产生了一个高分辨率的参考地图H2A.Z和散装核小体的位置在整个基因组的果蝇和比较，从酵母酿酒酵母。与酵母菌属一样，果蝇核小体围绕转录活性起始位点以典型的-1，无核小体区域，+1排列。然而，果蝇不将H2A.Z整合到-1核小体中，并且不将其转录起始位点埋在+1核小体中。在数千个基因中，RNA聚合酶II与+1核小体接合并暂停。转录起始机制如何与+1核小体竞争似乎在主要的真核细胞系中是根本不同的。
Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-free region, +1 arrangement. However, Drosophila does not incorporate H2A.Z into the -1 nucleosome and does not bury its transcriptional start site in the +1 nucleosome. At thousands of genes, RNA polymerase II engages the +1 nucleosome and pauses. How the transcription initiation machinery contends with the +1 nucleosome seems to be fundamentally different across major eukaryotic lines.