Isolation and characterization of marine bacterial strain degrading fucoidan from Korean Undaria pinnatifida Sporophylls.
Isolation and characterization of marine bacterial strain degrading fucoidan from Korean Undaria pinnatifida Sporophylls.
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韩国裙带菜孢子叶中降解岩藻依聚糖的海洋细菌菌株的分离和表征。
DOI：
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发表时间：
2008
影响因子：
2.8
通讯作者：
Y. Park
中科院分区：
文献类型：
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作者：
Woo;Sung;Y. Lee;H. Kim;Hyungjun Kim;S. Moon;H. Suh;K. Jang;Y. Park
尽管对岩藻聚糖作为生物活性化合物的兴趣越来越大，但还没有具有岩藻聚糖酶活性的方便的商业来源。从韩国裙带菜（Undaria pinnatifida）孢子叶中分离到一株在含有岩藻依聚糖作为唯一碳源的基本培养基上能融合生长的海洋细菌，经16 S rDNA序列分析鉴定为少动鞘氨醇单胞菌（Sphingomonaspaucimobilis），命名为少动鞘氨醇单胞菌PF-1。该菌株将岩藻依聚糖解聚成7种以上不同的低分子量含岩藻糖的寡糖，范围为305至3，749 Da。该酶活性显示与整个细胞相关，表明该酶可能存在于表面。然而，全细胞酶制剂既不从岩藻依聚糖中释放单体L岩藻糖，也不水解显色底物对硝基苯基-α-L-岩藻糖苷，表明该酶可能是内作用岩藻依聚糖酶而不是α-L-岩藻糖苷酶。因此，这似乎是第一次报告岩藻多糖分解活性的鞘氨醇单胞菌属物种，也是第一次报告酶降解的韩国裙带菜孢子叶岩藻多糖。此外，这种酶的活性可能是非常有用的含岩藻糖的多糖的结构分析和生物活性岩藻寡糖的生产。
In spite of an increasing interest in fucoidans as biologically active compounds, no convenient commercial sources with fucoidanase activity are yet available. A marine bacterial strain that showed confluent growth on a minimal medium containing fucoidan, prepared from Korean Undaria pinnatifida sporophylls, as the sole carbon source was isolated and identified based on a 16S rDNA sequence analysis as a strain of Sphingomonas paucimobilis, and named Sphingomonas paucimobilis PF-1. The strain depolymerized fucoidan into more than 7 distinct lowmolecular- mass fucose-containing oligosaccharides, ranging from 305 to 3,749 Da. The enzyme activity was shown to be associated with the whole cell, suggesting the possibility of a surface display of the enzyme. However, a whole-cell enzyme preparation neither released the monomer Lfucose from the fucoidan nor hydrolyzed the chromogenic substrate p-nitrophenyl-alpha-L-fucoside, indicating that the enzyme may be an endo-acting fucoidanase rather than an alpha-L-fucosidase. Therefore, this would appear to be the first report on fucoidanolytic activity by a Sphingomonas species and also the first report on the enzymatic degradation of the Korean Undaria pinnatifida sporophyll fucoidan. Moreover, this enzyme activity may be very useful for structural analyses of fucose-containing polysaccharides and the production of bioactive fucooligosaccharides.