Microchip separations in reduced-gravity and hypergravity environments
Microchip separations in reduced-gravity and hypergravity environments
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DOI：
10.1021/ac051198e
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发表时间：
2005-12-15
影响因子：
7.4
通讯作者：
Gonda, SR
中科院分区：
文献类型：
--
作者：
Culbertson, CT;Tugnawat, Y;Gonda, SR
微加工流体技术，例如芯片实验室设备，为在天基平台上进行化学和生物化学研究提供了许多有吸引力的特点。我们已经建造了一个便携式的，电池驱动的微流体平台，在太空飞行和发射中将经历的失重和超重力条件下进行了测试。该装置由一个微芯片、一个微芯片支架、两个0- 8kv高压电源、一个高压开关、一个固态二极管泵浦绿色激光器、一个光列、一个通道光倍增器和一个惯性质量测量单元组成，全部由一台笔记本电脑控制，由10节D-cell碱性电池供电。该装置在美国宇航局的失重研究飞机上进行了测试，重力水平与美国宇航局计划在载人和无人航天器上使用基于生物报告器的微芯片对空间和行星环境进行环境监测有关。在两次飞行过程中，四种氨基酸在各种重力环境下进行了834次快速电泳分离，包括零重力、火星重力、月球重力和类似1.8重力的环境。所有分离在12秒内完成，并自动分析。用内迁移标准校正后，迁移时间重现性均< 1%相对标准偏差。
Microfabricated fluidics technology, e.g., lab-on-a-chip devices, offers many attractive features for performing chemistry and biochemistry on space-based platforms. We have constructed a portable, battery-operated microfluidic platform that was tested under reduced gravity and hypergravity conditions that would be experienced in space flight and launch. This device consisted of a microchip, microchip holder, two 0-8-kV high-voltage power supplies, a high-voltage switch, a solid-state diode-pumped green laser, an optical train, a channel photo-multiplier, and an inertial mass measurement unit all under the control of a laptop computer and powered by 10 D-cell alkaline batteries. The unit was tested on NASA's reduced gravity research aircraft at gravity levels that are relevant to NASA's intended use of bioreporter-based microchips for environmental monitoring of space and planetary environments on manned and unmanned spacecraft. Over the course of two flights, 834 fast electrophoretic separations of four amino acids were performed under a variety of gravitational environments including zero-g, Martian-g, lunar-g, and similar to 1.8-g. All separations were performed in less than 12 s and automatically analyzed. After correction with an internal migration standard, the migration time reproducibilities were all < 1 % relative standard deviation.