INTERACTION OF ESTRADIOL AND HIGH-DENSITY LIPOPROTEINS ON PROLIFERATION OF THE HUMAN-BREAST CANCER CELL-LINE MCF-7 ADAPTED TO GROW IN SERUM FREE CONDITIONS
INTERACTION OF ESTRADIOL AND HIGH-DENSITY LIPOPROTEINS ON PROLIFERATION OF THE HUMAN-BREAST CANCER CELL-LINE MCF-7 ADAPTED TO GROW IN SERUM FREE CONDITIONS
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DOI：
10.1016/0006-291x(85)91847-9
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
BAYARD, F
中科院分区：
文献类型：
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作者：
JOZAN, S;FAYE, JC;BAYARD, F
研究了人乳腺癌细胞系MCF-7在不同培养条件下对雌二醇和他莫昔芬的反应性。将来自指数培养的细胞与来自融合培养（“融合对数”细胞）复制后初始周期的细胞进行比较。已经观察到雌二醇对氚化胸腺嘧啶掺入的刺激随着细胞密度的降低而降低，并且在无血清培养基条件下，“汇合对数”细胞在四个细胞周期内对雌激素无反应。另一方面，他莫昔芬或他莫昔芬与孕酮左炔诺孕酮联合治疗可以抑制从指数增长中复制的细胞的生长，以及从融合培养中复制的细胞。当细胞从指数增长的培养中复制时，这种生长抑制作用可以通过雌二醇来挽救。当细胞从融合培养中复制时，单独使用雌二醇（10-10 ~ 10-8M）不能恢复生长抑制作用。在这种情况下，添加类固醇耗尽的血清是必要的，以扭转雌二醇无反应的状态。血清可以用高密度脂蛋白代替，但不能用低密度脂蛋白或脂蛋白缺乏血清代替。目前的数据表明雌二醇和HDL在MCF-7细胞增殖的控制中相互作用。
The responsiveness of the human mammary carcinoma cell line MCF-7 to estradiol and tamoxifen treatment has been studied in different culture conditions. Cells from exponentially growing cultures were compared with cells in their initial cycles after replating from confluent cultures ("confluent-log" cells). It has been observed that estradiol stimulation of tritiated thymidine incorporation decreases with cell density and that "confluent-log" cells are estrogen unresponsive for a period of four cell cycles in serum-free medium conditions. On the other hand, growth of cells replated from exponentially growing, as well as from confluent cultures, can be inhibited by tamoxifen or a combined treatment with tamoxifen and the progestin levonorgestrel. This growth inhibitory effect can be rescued by estradiol when cells are replated from exponentially growing cultures. The growth inhibitory effect cannot be rescued by estradiol alone (10-10 to 10-8M) when cells are replated from confluent cultures. In this condition, the addition of steroid depleted serum is necessary to reverse the state of estradiol unresponsiveness. Serum can be replaced by high density lipoproteins but not by low density lipoproteins or lipoprotein deficient serum. The present data show that estradiol and HDL interact in the control of MCF-7 cell proliferation.