Characterization of a sabinene synthase gene from rough lemon (Citrus jambhiri)
Characterization of a sabinene synthase gene from rough lemon (Citrus jambhiri)
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DOI：
10.1016/j.jplph.2009.04.003
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Akimitsu, Kazuya
中科院分区：
文献类型：
--
作者：
Kohzaki, Keisuke;Gomi, Kenji;Akimitsu, Kazuya
我们之前从粗柠檬（Citrus jambhiri）中分离出两个假定的单萜合酶基因 RlemTPS1 和 RlemTPS2，并表明 RlemTPS2 的基因表达是由微生物攻击诱导的。利用原核表达系统获得RlemTPS2的蛋白产物，并通过RlemTPS2单萜合成的GC和GC-MS确定RlemTPS2编码桧烯合酶。 Sabinene 对链格孢 (Alternaria alternata) 具有抗真菌活性。此外，定点诱变鉴定出一种氨基酸，即位于金属前面的氨基酸。离子结合基序作为桧烯合酶产物特异性的重要残基。 (C) 2009 爱思唯尔有限公司。版权所有。
We previously isolated two putative monoterpene synthase genes, RlemTPS1 and RlemTPS2, from rough lemon (Citrus jambhiri) and showed that gene expression of RlemTPS2 was induced by microbial attack. The protein product of RlemTPS2 was obtained using a prokaryotic expression system, and GC and GC-MS of monoterpene synthesis by RlemTPS2 determined that RlemTPS2 encodes a sabinene synthase. Sabinene has antifungal activity toward Alternaria alternata. Furthermore, site-directed mutagenesis identified one amino acid, lie, located at the front of the metal. ion binding motif as an important residue for the product specificity of sabinene synthase. (C) 2009 Elsevier GmbH. All rights reserved.