Dynamic in vitro quantification of bioprosthetic heart valve leaflet motion using structured light projection
Dynamic in vitro quantification of bioprosthetic heart valve leaflet motion using structured light projection
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DOI：
10.1114/1.1415523
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发表时间：
2001-11-01
影响因子：
3.8
通讯作者：
Sacks, MS
中科院分区：
文献类型：
--
作者：
Iyengar, AKS;Sugimoto, H;Sacks, MS
心循环期间心脏瓣膜小叶变形的量化对于理解正常和病理瓣膜功能以及设计替代心脏瓣膜至关重要。由于涉及的技术复杂性，迄今为止对动态阀叶运动的研究很少。我们开发了一种新的实验方法，利用非接触结构激光投影技术来研究动态小叶运动。利用模拟的循环回路，150-200个激光光点的矩阵被投射到整个叶片表面。为了获得小叶表面的无障碍视图，使用高分辨率的立体系统来跟踪心脏周期中离散时间点的光点。每个时间点的小叶表面在三维中重建，并使用我们的双五次hermite有限元方法进行拟合(Smith et al., Ann。生物医学。工程学报。26:598-611,2001)。为了证明我们的方法，我们使用了一个牛心包生物人工心脏瓣膜，该瓣膜在打开和关闭阶段显示出复杂的弯曲变形区域和本质上不同的形状。综上所述，该方法具有较高的空间和时间分辨率，可以同时重建整个尖端表面。因为它是完全非接触的，这种方法适用于疲劳研究和组织工程心脏瓣膜的生物反应器技术。(C) 2001生物医学工程学会。
Quantification of heart valve leaflet deformation during the cardiac cycle is essential in understanding normal and pathological valvular function, as well as in the design of replacement heart valves. Due to the technical complexities involved, little work to date has been performed on dynamic valve leaflet motion. We have developed a novel experimental method utilizing a noncontacting structured laser-light projection technique to investigate dynamic leaflet motion. Using a simulated circulatory loop, a matrix of 150-200 laser light points were projected over the entire leaflet surface. To obtain unobstructed views of the leaflet surface, a stereo system of high-resolution boroscopes was used to track the light points at discrete temporal points during the cardiac cycle. The leaflet surface at each temporal point was reconstructed in three dimensions, and fit using our biquintic hermite finite element approach (Smith et al., Ann. Biomed. Eng. 26:598-611, 2001). To demonstrate our approach, we utilized a bovine pericardial bioprosthetic heart valve, which revealed regions of complex flexural deformation and substantially different shapes during the opening and closing phases. In conclusion, the current method has high spatial and temporal resolution and can reconstruct the entire surface of the cusp simultaneously. Because it is completely noncontacting, this approach is applicable to studies of fatigue and bioreactor technology for tissue engineered heart valves. (C) 2001 Biomedical Engineering Society.