Glutathiyl radical as an intermediate in glutathione nitrosation.
Glutathiyl radical as an intermediate in glutathione nitrosation.
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谷胱甘肽自由基作为谷胱甘肽亚硝化的中间体。
DOI：
10.1016/j.freeradbiomed.2012.08.013
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发表时间：
2012
影响因子：
7.4
通讯作者：
Tsoukias,NikolaosM
中科院分区：
文献类型：
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作者：
Madrasi,Kumpal;Joshi,MaheshS;Gadkari,Tushar;Kavallieratos,Konstantinos;Tsoukias,NikolaosM
硫醇的亚硝化被认为是由三氧化二氮(N2O)或二氧化氮自由基(NO2)介导的。研究了在充气缓冲溶液中NO供体亚硝化谷胱甘肽(GSH)的动力学。用分光光度法和化学发光法测定了S-亚硝基谷胱甘肽(GSnO)的形成。结果表明，GSNO的生成速率随GSH的增加而增加，其EC50的一半最大值依赖于NO浓度。我们观察到的EC50随着NO浓度的增加而增加，这表明NO2介导的亚硝化反应在GSNO的产生中起着重要的作用，谷胱甘肽作为中间体。
Nitrosation of thiols is thought to be mediated by dinitrogen trioxide (N2O3) or by nitrogen dioxide radical (NO2). A kinetic study of glutathione (GSH) nitrosation by NO donors in aerated buffered solutions was undertaken. S-nitrosoglutathione (GSNO) formation was assessed spectrophotometrically and by chemiluminescence. The results suggest an increase in the rate of GSNO formation with an increase in GSH with a half-maximum constant EC50that depends on NO concentration. Our observed increase in EC50with NO concentration suggests a significant contribution of NO2-mediated nitrosation with the glutathiyl radical as an intermediate in the production of GSNO.