Genetic mapping and chromosome localization of the rat mitochondrial glycerol-3-phosphate dehydrogenase gene, a candidate for non-insulin-dependent diabetes mellitus.
Genetic mapping and chromosome localization of the rat mitochondrial glycerol-3-phosphate dehydrogenase gene, a candidate for non-insulin-dependent diabetes mellitus.
复制标题
大鼠线粒体甘油-3-磷酸脱氢酶基因的遗传图谱和染色体定位，该基因是非胰岛素依赖型糖尿病的候选基因。
DOI：
10.1006/geno.1996.0599
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发表时间：
1996
期刊：
影响因子：
4.4
通讯作者：
Jacob,HJ
中科院分区：
文献类型：
--
作者：
Koike,G;VanVooren,P;Shiozawa,M;Galli,J;Li,LS;Glaser,A;Balasubramanyam,A;Brown,LJ;Luthman,H;Szpirer,C;MacDonald,MJ;Jacob,HJ
线粒体 FAD 依赖性甘油 3-磷酸脱氢酶 (mtGPD) 在胰岛素分泌调节中发挥重要作用，并被认为是人类非胰岛素依赖性糖尿病 (NIDDM) 以及 NIDDM 啮齿动物模型发病机制的候选者。最近对 NIDDM Goto-Kakizaki (GK) 大鼠模型的分子遗传学研究已经确定了与 NIDDM 相关的基因座。为了阐明大鼠mtGPD是否在NIDDM的发病机制中发挥作用，克隆了大鼠mtGPD基因(Gpd2)，并开发了Gpd2的遗传标记。该基因定位到大鼠 3 号染色体区域，该区域包含与 GK 大鼠中的 NIDDM 相关的区域。还进行了荧光原位杂交以验证图谱位置。
The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (mtGPD) plays an important role in the regulation of insulin secretion and has been postulated as a candidate responsible for the pathogenesis of non-insulin-dependent diabetes mellitus (NIDDM) in humans as well as in rodent models of NIDDM. Recent molecular genetic studies of the Goto–Kakizaki (GK) rat model of NIDDM have identified loci linked to NIDDM. To elucidate whether rat mtGPD might play a role in the pathogenesis of NIDDM, the rat mtGPD gene (Gpd2) was cloned, and a genetic marker forGpd2was developed. The gene mapped to the region of rat chromosome 3 that contains a region linked to NIDDM in the GK rat. Fluorescencein situhybridization was also carried out to verify the map position.