Occurrence of RD9 region and 500 bp fragment among clinical isolates of Mycobacterium tuberculosis and Mycobacterium bovis
Occurrence of RD9 region and 500 bp fragment among clinical isolates of Mycobacterium tuberculosis and Mycobacterium bovis
复制标题
DOI：
10.1111/j.1348-0421.2007.tb03905.x
复制
发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Verma, Rishendra
中科院分区：
文献类型：
--
作者：
Das, Samir;Das, Suresh Chandra;Verma, Rishendra
本研究涉及结核分支杆菌和M.牛RD9区和500 bp片段PCR检测。八米结核病5例，M.从40份人痰标本、41份牛肺标本和20份M.结核病和M.本研究包括在印度兽医研究所分枝杆菌实验室保存的牛菌株。这样的话，28M。结核病和14例M. bovis菌株和M. tuberculosis H37Rv，M. bovis BCG、M. canetti，M. sinegalus，M. phlei，M. chelonae、龟类M. kansasii，M. xenopi和M.用RD9和500 bp的PCR扩增。所有的M。结核菌株、M.结核H37 Rv和M. canetti扩增产物为333 bp，表明这些菌株中存在RD9区，而所有M.经RD9 PCR扩增，扩增产物为206 bp。在M. bovis BCG、M. xenopi，M. smegalgae，M. phlei，M. chelonae、龟类M. kansasii和M. avium PCR扩增结果显示，所有M. bovis菌株和M.牛卡介苗在M. canetti，M. smegalgae，M. phlei，M. chelonae、龟类M. avium，M. kansasii，M. xenopi，在所有M.结核菌株PCR检测结果与分离株的培养和生化结果的相关性为100%。我们的研究表明，基于RD9和500 bp的PCR可以有效地鉴定两种密切相关的M。结核和M.牛
The present investigation dealt with the identification of Mycobacterium tuberculosis and M. bovis by RD9 region and 500 bp fragment PCR assays. Eight M. tuberculosis and 5 M. bovis characterized and identified from 40 human sputum and 41 bovine lung specimens and 20 M. tuberculosis and 9 M. bovis strains maintained at Mycobacteria Laboratory, Indian Veterinary Research Institute were included in this study. In this way, 28 M. tuberculosis and 14 M. bovis strains and, for comparison and control purpose, M. tuberculosis H37Rv, M. bovis BCG, M. canetti, M. sinegmatis, M. phlei, M. chelonae, M. kansasii, M. xenopi and M. avium were subjected to RD9 and 500 bp amplification by PCR. All M. tuberculosis strains, M. tuberculosis H37 Rv and M. canetti yielded a product of 333 bp which showed presence of RD9 region in these strains, whereas all M. bovis yielded a product of 206 bp with RD9 PCR assay. There was no amplification product found in M. bovis BCG, M. xenopi, M. smegmatis, M. phlei, M. chelonae, M. kansasii, and M. avium. PCR based on 500 bp fragment showed a product of 500 bp in all M. bovis strains and M. bovis BCG. There was no amplification product of 500 bp found in M. canetti, M. smegmatis, M. phlei, M. chelonae, M. avium, M. kansasii, M. xenopi and was absent in all M. tuberculosis strains. The PCR assay results correlated 100% with the culture and biochemical results of the isolates. Our study suggested that PCR based on RD9 and 500 bp may effectively identify two closely related species of M. tuberculosis and M. bovis.