Viability and antibacterial efficacy of four root canal disinfection techniques evaluated using confocal laser scanning microscopy.
Viability and antibacterial efficacy of four root canal disinfection techniques evaluated using confocal laser scanning microscopy.
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DOI：
10.4103/0972-0707.139833
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发表时间：
2014-09-01
期刊：
影响因子：
--
通讯作者：
Mathew, Jean
中科院分区：
文献类型：
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作者：
Mathew, Joan;Emil, Jonathan;Mathew, Jean
背景：最近推出了几种消毒技术，主要目的是改善根管系统难以到达区域的根管消毒。这项体外研究的目的是使用传统冲洗、EndoActivator（登士柏，塔尔萨牙科诊所，美国）、二极管激光照射和光子引发光声流 (PIPS) 来评估粪肠球菌生物膜的抗菌效果和活力。 材料和方法：对 130 个单根下颌前磨牙的根管进行仪器测量，标准化为 20 毫米的统一长度，直到完成文件， F1（通用 Protaper 旋转系统，登士柏，塔尔萨牙科专业公司，美国）。去除涂污层并消毒后，随机选取5颗牙齿，确保无菌后进行细菌接种。其余 125 个样本被粪肠球菌悬浮液污染，孵育 21 天并分为 5 组（n = 25）。在第 1 组；未治疗组（阳性对照），根管不进行任何消毒程序。在根管内进行取样，并评估 20 个样品的菌落形成单位计数。选择五个样本，通过共焦激光扫描显微镜观察 1 级（距顶点 4 毫米）和 2 级（距顶点 1 毫米）的定植模式。第 2-5 组中的样品，即传统针冲洗、EndoActivator、二极管激光和 PIPS 进行了各自的消毒程序。所有组的消毒后样品评估标准均与第 1 组相同。结果：与其他三种消毒技术相比，二极管激光显示出最高的抗菌功效和最少的活菌数。结论：在最小化仪器、实验感染的根管中，与传统针冲洗、PIPS 和 EndoActivator 组相比，二极管激光组显示出更好的抗菌功效和最少的活菌数。
BACKGROUND: Several disinfection techniques have been recently introduced with the main objective of improving root canal disinfection in the inaccessible areas of the root canal system. This in vitro study was done to evaluate the antimicrobial effect and viability of Enterococcus faecalis biofilms using conventional irrigation, EndoActivator (Dentsply, Tulsa Dental, USA), diode laser irradiation and photon-initiated photoacoustic streaming (PIPS).MATERIALS AND METHODS: Root canals of 130 single rooted mandibular premolars, standardized to a uniform length of 20 mm were instrumented until finishing file, F1 (Universal Protaper Rotary System, Dentsply, Tulsa Dental Specialties, USA). After smear layer removal and sterilization, five teeth were randomly selected to assure sterility before bacterial inoculation. The remaining 125 samples were contaminated with E. faecalis suspension, incubated for 21 days and divided into five groups (n = 25). In Group 1; untreated group (positive control), the root canals were not subjected to any disinfection procedure. Sampling was performed within the canals and the colony-forming unit count was evaluated for 20 samples. Five samples were selected to visualize the pattern of colonization at Level 1 (4 mm from the apex) and Level 2 (1 mm from the apex) by confocal laser scanning microscopy. Samples in Groups 2-5 namely conventional needle irrigation, EndoActivator, diode laser and PIPS were subjected to their respective disinfection procedures. Postdisinfection sample evaluation criteria was followed for all groups as same as that for Group 1.RESULTS: Diode laser displayed the highest antibacterial efficacy and least viable bacteria than the other three disinfection techniques.CONCLUSION: Diode laser group showed better antibacterial efficacy and least viable bacteria when compared to conventional needle irrigation, PIPS and EndoActivator groups in minimally instrumented, experimentally infected root canals.