Preparation of collagen scaffolds and their applications in tissue engineering.
Preparation of collagen scaffolds and their applications in tissue engineering.
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DOI：
10.1515/bmte.2002.47.s1a.485
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发表时间：
2002-01-01
期刊：
影响因子：
--
通讯作者：
Rau, G
中科院分区：
文献类型：
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作者：
Kuberka, M;Heschel, I;Rau, G
冷冻干燥的胶原蛋白支架可用于各种医学组织工程应用。支架的孔结构对细胞的接种、生长和分化起着决定性的作用。对于受控的3D细胞生长，孔结构需要是均匀的，并且孔尺寸可以单独调节。对于冻干支架，孔结构由在稳定条件下冷冻期间的冰晶形态决定。达到了具有均匀孔结构和25至100微米的孔径范围的支架。细胞如前脂肪细胞、角质形成细胞和成纤维细胞显示良好地粘附于胶原基质。
Freeze-dried collagen scaffolds can be used for a variety of medical tissue engineering applications. The pore structure of the scaffolds might play a decisive role for the inoculation, growth and differentiation of the cells. For a controlled 3D-cell growth the pore structure needs to be homogeneous and the pore size individually adjustable. For lyophilised scaffolds, the pore structure is determined by the ice crystal morphology during freezing under steady conditions. Scaffolds with a homogeneous pore structure and a range of pore size between 25 and 100 microns were reached. Cells such as preadipocytes, keratinocytes, and fibroblasts showed to adhere well to the collagen matrix.