Comparison of modified chandler, roller pump, and ball valve circulation models for in vitro testing in high blood flow conditions: application in thrombogenicity testing of different materials for vascular applications.
Comparison of modified chandler, roller pump, and ball valve circulation models for in vitro testing in high blood flow conditions: application in thrombogenicity testing of different materials for vascular applications.
复制标题
DOI：
10.1155/2012/673163
复制
发表时间：
2012
影响因子：
3.1
通讯作者：
de Hart J
中科院分区：
文献类型：
--
作者：
van Oeveren W;Tielliu IF;de Hart J
三种不同的模型，一个修改的钱德勒环路，滚子泵，和一个新的球阀模型（Hemorrhage），进行了比较方面的内在损害的血液成分和血小板活化。Hemorrhage用于测试聚合物管。 钱德勒环路不可能实现高流量。滚子泵和Hemorrhage可以调节到高流量，但泵诱导溶血。滚子泵和钱德勒袢中血小板数量减少（P < 0.05），但Hemorrhage中仍较高。血小板聚集在所有模型中均减少。 Hemorrhage用于测试血管移植物材料，并允许不同的回路在37°C下同时循环。ePTFE、Dyneema Purity UHMWPE纤维和PET纤维管均显示溶血低于0.2%，血小板计数和功能降低。PET上的纤维蛋白和血小板结合较高，Dyneema Purity UHMWPE上的炎症标记物最低。 我们得出结论，Hemorrhage对血液的影响最小，可以调整为高血流量，模拟动脉剪切应力。Hemorrhage用于测量移植物材料的血液相容性，并显示Dyneema Purity UHMWPE纤维在许多方面比ePTFE和PET更具有血液相容性。
Three different models, a modified Chandler loop, roller pump, and a new ball valve model (Hemobile), were compared with regard to intrinsic damage of blood components and activation of platelets. The Hemobile was used for testing of polymer tubes. High flow was not possible with the Chandler loop. The roller pump and the Hemobile could be adjusted to high flow, but he pump induced hemolysis. Platelet numbers were reduced in the roller pump and Chandler loop (P < 0.05), but remained high in the Hemobile. Platelet aggregation was reduced in all models. The Hemobile was applied for testing vascular graft materials, and allowed different circuits circulated simultaneously at 37°C. ePTFE, Dyneema Purity UHMWPE fiber and PET fiber based tubes, all showed hemolysis below 0.2% and reduced platelet count and function. Binding of fibrin and platelets was higer on PET, inflammatory markers were lowest on Dyneema Purity UHMWPE. We concluded that the Hemobile minimally affects blood and could be adjusted to high blood flows, simulating arterial shear stress. The Hemobile was used to measure hemocompatibility of graft material and showed Dyneema Purity UHMWPE fiber in many ways more hemocompatible than ePTFE and PET.