Changes in DNA Polymerase Activities in Pupae of the Silkworm Bombyx mori after Infection with Nuclear Polyhedrosis Virus
Changes in DNA Polymerase Activities in Pupae of the Silkworm Bombyx mori after Infection with Nuclear Polyhedrosis Virus
复制标题
家蚕蛹感染核型多角体病毒后DNA聚合酶活性的变化
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
P. Kullyev
中科院分区：
文献类型：
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作者：
V. Mikhailov;J. O. Ataeva;K. A. Marlyev;P. Kullyev
总结
本文研究了家蚕蛹感染核型多角体病毒（BmNPV）后DNA聚合酶活性的变化。雌雄蛹接种BmNPV后，细胞内α-聚合酶活性显著增加，并诱导产生一种新的DNA聚合酶（BmNPV-polymerase）。感染蛹中α和BmNPV的DNA聚合酶活性呈平行上升趋势，72 h达最大值，随后下降。病毒感染不影响蛹中γ-聚合酶的活性。在蛹死亡之前的感染周期的后期，检测到蛹中的β-聚合酶活性增加。DNA聚合酶在BmNPV复制中的可能作用进行了讨论。
Summary
Changes in DNA polymerase activities were studied in pupae of the silkworm Bombyx mori upon infection with nuclear polyhedrosis virus (BmNPV). Two effects were observed in male and female pupae after inoculation of BmNPV: a marked increase in cellular α-polymerase activity and the induction of a new DNA polymerase (BmNPV-polymerase). Activities of both DNA polymerases, α and BmNPV, in the infected pupae increased in parallel, reached a maximum at 72 h post-inoculation and decreased subsequently. The virus infection did not affect the activity of γ-polymerase in the pupae. An increased activity of β-polymerase in the pupae was detected at the late stage of the infection cycle prior to pupal death. The possible roles of the DNA polymerases in BmNPV replication are discussed.