Haemagglutination patterns of Helicobacter pylori
Haemagglutination patterns of Helicobacter pylori
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幽门螺杆菌的血凝模式
DOI：
10.1111/j.1699-0463.1992.tb04018.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
T. Wadström
中科院分区：
文献类型：
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作者：
J. Lelwala‐Guruge;A. Ljungh;T. Wadström
对32株幽门螺杆菌菌株与11种不同动物的红细胞进行了血凝试验。23株(72%)凝集人红细胞的菌株表现出广谱的HA图谱。高唾液酸偶联物的人、豚鼠和牛红细胞能被大多数菌株强烈凝集。除两株外，7株(22%)不凝集人红细胞的菌株表现出窄谱HA图谱，通常不能被唾液酸偶联物或N-乙酰神经氨基乳糖(NANLac)居住。将菌株分为三个主要的HA类别。I类菌株中有10株(31%)可被不同组合的NaNLac、类粘蛋白或胎球蛋白抑制，但不能被asialofetuin抑制，提示唾液酸专一性的存在可能识别NeuAcα-(2-3)-Gal异构体。II类菌株中有12株(38%)对不同组合的NANLac、类粘蛋白和胎球蛋白以及asialofetuin表现出不同的受体特异性结合。在III类中，有10株(31%)未观察到抑制作用；因此，该受体似乎不同于其他两类。在21株(66%)I、II级菌株中，11株(34%)对HA有抑制作用，14株(44%)对类口腔粘液有抑制作用，15株(47%)对胎儿蛋白有抑制作用。在HA模式中观察到的巨大异质性表明，不同菌株的HA可能识别红细胞膜上的一类不同类别的唾液糖结合物。
Thirty‐two Helicobacter pylori strains were screened for haemagglutination (HA) activity with erythrocytes of 11 different animal species. Twenty‐three strains (72%) that agglutinated human erythrocytes exhibited a broad‐spectrum HA profile. Human, guinea pig and bovine erythrocytes high in sialoglycoconjugates were strongly agglutinated by most strains. Except for two, seven strains (22%) that did not agglutinate human erythrocytes exhibited a narrow‐spectrum HA profile, commonly not inhabitable by sialoglycoconjugates or N‐acetylneuraminlactose (NANLac). Strains were classified into three major HA classes. HA of 10 strains (31%) in class I was inhibited by different combinations of NANLac, orosomucoid or fetuin, but not by asialofetuin, suggesting the presence of sialic acid‐specific HAs probably recognizing NeuAc α‐(2–3)‐ Gal isomer. Twelve strains (38%) in class II exhibited a different receptor specificity binding to different combinations of NANLac, orosomucoid and fetuin, as well as asialofetuin. No inhibition was observed with 10 strains (31%) in class III; thus, this receptor seems different from both the other classes. Of 21 strains (66%) in classes I and II, HA of 11 strains (34%) was inhibited with NANLac, 14 strains (44%) with orosomucoid and 15 strains (47%) with fetuin. The great heterogeneity observed in HA patterns indicates that the HAs of different strains may recognize a heterogeneous class of sialoglycoconjugates on the erythrocyte membrane.