Isolation and characterization of six peach cDNAs encoding key proteins in organic acid metabolism and solute accumulation: involvement in regulating peach fruit acidity
Isolation and characterization of six peach cDNAs encoding key proteins in organic acid metabolism and solute accumulation: involvement in regulating peach fruit acidity
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DOI：
10.1034/j.1399-3054.2002.1140212.x
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发表时间：
2002-02-01
影响因子：
6.4
通讯作者：
Rothan, C
中科院分区：
文献类型：
--
作者：
Etienne, C;Moing, A;Rothan, C
与其它许多肉质果实一样，成熟桃（Prunus persica（L.）Batsch）水果是苹果酸和柠檬酸。这些代谢物在果肉中的积累在果实发育过程中受到调节。克隆了6个与桃果实有机酸代谢（线粒体柠檬酸合成酶、胞质NAD依赖的苹果酸脱氢酶和胞质NADP依赖的异柠檬酸脱氢酶）和贮藏（液泡质子转运泵：1个液泡H+-ATP酶和2个液泡H+-焦磷酸酶）相关的基因。其中五个桃基因与从其他肉质水果物种的果实中分离的基因同源。系统发育和表达分析表明，存在一个特定的液泡焦磷酸酶在水果中高度表达。第六个基因是第一个从果实中分离的胞质NAD依赖的苹果酸脱氢酶基因。研究了两个桃品种，正常酸（幻想曲）和低酸（Jalousia）品种果实发育过程中的基因表达。两个品种的有机酸相关基因的总体表达模式出现惊人的相似。与有机酸代谢相关的基因在果实成熟期的表达较发育早期强，但其表达模式与有机酸含量的变化不一定相关。液泡膜质子泵的双相表达模式与有机酸积累的模式更一致，并且液泡膜焦磷酸酶在果实的第二快速生长期的低酸品种的果实中更高地表达。
As in many other fleshy fruits, the predominant organic acids in ripe peach (Prunus persica (L.) Batsch) fruit are malic and citric acids. The accumulation of these metabolites in fruit flesh is regulated during fruit development. Six peach fruit-related genes implicated in organic acid metabolism (mitochondrial citrate synthase; cytosolic NAD-dependent malate dehydrogenase, and cytosolic NADP-dependent isocitrate dehydrogenase) and storage (vacuolar proton translocating pumps: one vacuolar H+-ATPase, and two vacuolar H+-pyrophosphatases) were cloned. Five of these peach genes were homologous to genes isolated from fruit in other fleshy fruit species. Phylogenetic and expression analyses suggested the existence of a particular vacuolar pyrophosphatase highly expressed in fruit. The sixth gene was the first cytosolic NAD-dependent malate dehydrogenase gene isolated from fruit. Gene expression was studied during the fruit development of two peach cultivars, a normal-acid (Fantasia) and a low-acid (Jalousia) cultivar. The overall expression patterns of the organic acid-related genes appeared strikingly similar for the two cultivars. The genes involved in organic acid metabolism showed a stronger expression in ripening fruit than during the earlier phases of development, but their expression patterns were not necessarily correlated with the changes in organic acid contents. The tonoplast proton pumps showed a biphasic expression pattern more consistent with the patterns of organic acid accumulation, and the tonoplast pyrophosphatases were more highly expressed in the fruit of the low-acid cultivar during the second rapid growth phase of the fruit.