Multiprefectural Spread of Gastroenteritis Outbreaks Attributable to a Single Genogroup II Norovirus Strain from a Tourist Restaurant in Nagasaki, Japan
Multiprefectural Spread of Gastroenteritis Outbreaks Attributable to a Single Genogroup II Norovirus Strain from a Tourist Restaurant in Nagasaki, Japan
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DOI：
10.1128/jcm.43.3.1093-1098.2005
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发表时间：
2005-03
影响因子：
9.4
通讯作者：
Y. Hirakata;K. Arisawa;O. Nishio;O. Nakagomi
中科院分区：
文献类型：
--
作者：
Y. Hirakata;K. Arisawa;O. Nishio;O. Nakagomi
摘要 2003年11月18日和19日，来自日本几个县的旅游团中发生了一系列由诺如病毒（NVs）引起的胃肠炎暴发，这些暴发与食用日本长崎市一家餐厅准备的午餐有关。进行了一项回顾性队列研究，以评估暴发的规模并确定感染源。通过疾病发生地区的当地公共卫生中心获取流行病学信息。使用基因群特异性引物通过逆转录 - 聚合酶链反应对粪便和呕吐物标本以及食品和环境样本进行分析。对阳性样本进行测序并进行系统发育分析。在两天期间食用了该餐厅准备的午餐的1492名游客中，660人（44.2%）发病，平均潜伏期为31.2小时。虽然在任何食品样本中均未检测到诺如病毒，但在病例患者、餐厅工作人员和厨房餐桌的标本中发现了与基因群II诺如病毒墨西哥基因型最密切相关的相同序列。由于几餐食物受到污染，得出食品加工人员是此次暴发的源头。此处描述的一系列暴发例证了旅游业作为一种将单一传染因子传播到多个地理上偏远地区的当代途径的作用。
ABSTRACT A series of gastroenteritis outbreaks caused by noroviruses (NVs) among tourist groups from several prefectures was associated with eating a lunch prepared by a restaurant in Nagasaki City, Japan, on 18 and 19 November 2003. A retrospective cohort study was performed to estimate the magnitude of the outbreak and identify the source of infection. Epidemiological information was obtained through the local public health centers in the areas where the illness occurred. Stool and vomit specimens and food and environmental samples were analyzed by reverse transcription-PCR with genogroup-specific primers. Positive samples were sequenced and analyzed phylogenetically. Of 1,492 tourists who ate a lunch prepared by the restaurant during the 2-day period, 660 (44.2%) developed illness, with an average incubation time of 31.2 h. Whereas NVs were not detected in any food samples, identical sequences most closely related to the Mexico genotype of genogroup II NV were found in specimens from case patients, restaurant staff, and the kitchen table. Food handlers were concluded to be the source of the outbreak as a result of the contamination of several meals. The series of outbreaks described here exemplifies the role of tourism as a contemporary way to distribute a single infectious agent to multiple and geographically remote areas.