ANTIBODY-DEPENDENT ENHANCEMENT OF DENGUE VIRUS GROWTH IN HUMAN-MONOCYTES AS A RISK FACTOR FOR DENGUE HEMORRHAGIC-FEVER
ANTIBODY-DEPENDENT ENHANCEMENT OF DENGUE VIRUS GROWTH IN HUMAN-MONOCYTES AS A RISK FACTOR FOR DENGUE HEMORRHAGIC-FEVER
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DOI：
10.4269/ajtmh.1989.40.444
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发表时间：
1989-04-01
影响因子：
3.3
通讯作者：
BURKE, DS
中科院分区：
文献类型：
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作者：
KLIKS, SC;NISALAK, A;BURKE, DS
在曼谷[泰国]学童登革热感染的一项前瞻性研究中收集的血清标本在体外测试了它们促进登革热2型病毒在人单核细胞中的生长的能力。比较两组登革热免疫血清：随后发生无症状继发性登革热感染的32例登革热抗体阳性儿童血清标本；随后出现严重症状继发性登革热感染的儿童登革热抗体阳性血清标本9例，其中8例临床诊断为登革出血热。在未稀释的试验血清存在的情况下，通过测量感染了DEN-2病毒的正常人单核细胞培养物的上清液中的病毒产量，来定量测定抗体依赖性病毒生长的增强。32例无症状儿童感染前血清中仅有4例（12%）具有显著增强活性，而9例有症状儿童感染前血清中有6例（67%）具有显著增强活性（P < 0.001）。高血清DEN-2抗体依赖性增强活性是登革热出血热流行地区儿童严重疾病的重要危险因素（相对危险度= 6.2）。登革热抗体可以起到中和作用，因此具有保护作用，也可以增强并增加登革出血热的风险。
Serum specimens collected during a prospective study for dengue infections among schoolchildren in Bangkok [Thailand] were tested for their ability to enhance dengue 2 (DEN-2) virus growth in human monocytes in vitro. Two groups of dengue-immune sera were compared: 32 dengue antibody positive serum specimens from children who subsequently developed asymptomatic secondary dengue infections; and 9 dengue antibody positive serum specimens from children who subsequently developed severe symptomatic secondary dengue infections, 8 of which were clinically diagnosed as dengue hemorrhagic fever. Antibody-dependent enhancement of virus growth was quantitated by measurement of virus yields in supernatant fluids of normal human monocyte cultures that were infected with DEN-2 virus in the presence of undiluted test serum. Only 4 of 32 (12%) preinfection sera from asymptomatic children, but 6 of 9 (67%) preinfection sera from symptomatic children, had significant enhancing activity (P < 0.001). High serum DEN-2 antibody dependent enhancing activity is a significant (relative risk = 6.2) risk factor for severe illness among children in a dengue hemorrhagic fever endemic region. Dengue antibodies can be neutralizing and therefore protective, or they can be enhancing and increase the risk of dengue hemorrhagic fever.