Smart optical probes for near-infrared fluorescence imaging of Alzheimer's disease pathology
Smart optical probes for near-infrared fluorescence imaging of Alzheimer's disease pathology
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DOI：
10.1007/s00259-007-0708-7
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发表时间：
2008-03-01
影响因子：
9.1
通讯作者：
Bacskai, Brian J.
中科院分区：
文献类型：
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作者：
Raymond, Scott B.;Skoch, Jesse;Bacskai, Brian J.
目的淀粉样β蛋白（A β）的近红外荧光探针是阿尔茨海默病研究中分子成像的一个令人兴奋的选择，并可能转化为临床诊断。然而，A β靶向的光学探针通常具有较差的特异性和缓慢的脑清除。方法合成了一系列的荧光染料，并通过荧光光谱和组织染色测试了它们与A β的结合灵敏度。结果所选化合物的荧光量子产率、荧光寿命、荧光强度和荧光强度均随A β的变化而变化。和发射光谱，可以在显微镜下或在体内成像，使用新的寿命和光谱荧光成像技术。与A β结合将改善淀粉样蛋白检测，并可能使体内定量分子成像成为可能。
Purpose Near-infrared fluorescent probes for amyloid-beta (A beta) are an exciting option for molecular imaging in Alzheimer's disease research and may translate to clinical diagnostics. However, A beta-targeted optical probes often suffer from poor specificity and slow clearance from the brain. We are designing smart optical probes that emit characteristic fluorescence signal only when bound to A beta.Methods We synthesized a family of dyes and tested A beta-binding sensitivity with fluorescence spectroscopy and tissue-staining.Results Select compounds exhibited A beta-dependent changes in fluorescence quantum yield, lifetime, and emission spectra that may be imaged microscopically or in vivo using new lifetime and spectral fluorescence imaging techniques.Conclusion Smart optical probes that turn on when bound to A beta will improve amyloid detection and may enable quantitative molecular imaging in vivo.