Inheritance of Permethrin Resistance in Culex quinquefasciatus
Inheritance of Permethrin Resistance in Culex quinquefasciatus
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DOI：
10.1603/me10142
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发表时间：
2010-11-01
影响因子：
2.1
通讯作者：
Liu, Nannan
中科院分区：
文献类型：
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作者：
Li, Ting;Liu, Nannan
在HAmCq(G0)、MAmCq(G0)和BAmCq(G0)这3种田间抗药菌株中研究了氯菊酯对致倦库蚊(Say)不同生命阶段的毒性；两种氯菊酯选择菌株，HAmCq(G8) 和 MAmCq(G6)；以及实验室敏感菌株 S-Lab。库蚊幼虫在不同发育阶段对氯菊酯的耐受水平存在差异，从一龄到四龄依次递增。库蚊幼虫中氯菊酯抗性的发育调节模式被确定，从一龄到四龄再次增加。与幼虫阶段相比，成年蚊子的抵抗力总体上相对较低，这表明幼虫和成虫之间对杀虫剂选择压力的反应可能涉及不同的机制。使用抗性 HAmCq(G8) 和易感 S-Lab 菌株之间的相互杂交来检查氯菊酯抗性的遗传； F-1倒数品系的对数浓度-概率响应曲线与敏感品系的对数浓度-概率响应曲线相似，显性度值为-0.2至-0.3，表明Cx具有氯菊酯抗性的遗传性。 quinquefasciatus 是不完全隐性的。 F-1 倒数品系之间的 LC50 值和对数浓度概率线的斜率没有发现显着差异，表明对氯菊酯的抗性是常染色体遗传的。回交和自交后代对氯菊酯反应的chi(2)拟合优度检验和对数浓度概率线分析均表明HAmCq(G8)品系中的氯菊酯抗性不遵循单基因基因遗传模型，揭示了Cx中的氯菊酯抗性。 quinquefasciatus 受多个基因控制。
The toxicity of permethrin to different life stages of Culex quinquefasciatus (Say) was examined in three field insecticide resistant strains, HAmCq(G0), MAmCq(G0), and BAmCq(G0); two permethrin selected strains, HAmCq(G8) and MAmCq(G6); and a laboratory susceptible strain, S-Lab. The levels of tolerance to permethrin differed among the developmental stages of Culex mosquito larvae with an ascending order from first to fourth instar. A developmentally regulated pattern of permethrin resistance in Culex mosquito larvae was identified, once again increasing from first to fourth instar. Adult mosquitoes had relatively lower levels of resistance in general compared with their larval stages, suggesting that different mechanisms may be involved in the response to insecticide selection pressure between larvae and adults. Inheritance of resistance to permethrin was examined using reciprocal crosses between resistant HAmCq(G8) and susceptible S-Lab strains; the resulting logarithm concentration-probit response curves for reciprocal F-1 lines were similar to those for the susceptible strain, with degree of dominance values of -0.2 to -0.3, suggesting that the inheritance of permethrin resistance in Cx. quinquefasciatus is incompletely recessive. No significant difference was found in the values of LC50 and the slope of the log concentration-probit lines between reciprocal F-1 lines, indicating that the resistance to permethrin was inherited autosomally. Both chi(2) goodness-of-fit test and log concentration-probit line analysis on responses of backcross and self-bred progeny to permethrin indicated that permethrin resistance in the HAmCq(G8) strain did not follow a monogenic gene inheritance model, revealing that permethrin resistance in Cx. quinquefasciatus is controlled by more than one gene.