THE CHEMICAL-STRUCTURE OF BACTERIAL-ENDOTOXIN IN RELATION TO BIOACTIVITY
THE CHEMICAL-STRUCTURE OF BACTERIAL-ENDOTOXIN IN RELATION TO BIOACTIVITY
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DOI：
10.1016/s0171-2985(11)80338-4
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发表时间：
1993-04-01
期刊：
影响因子：
2.8
通讯作者：
ZAHRINGER, U
中科院分区：
文献类型：
--
作者：
RIETSCHEL, ET;KIRIKAE, T;ZAHRINGER, U
内毒素是革兰氏阴性菌的O-抗原和内毒素。虽然内毒素的多糖和脂类部分都有助于这类细菌的致病潜力，但决定内毒素性质的是脂类成分(脂类A)。阐明了各种细菌来源的类脂A的一级结构，并用化学方法合成了大肠杆菌类脂A。合成类脂A部分结构的生物学分析证明，内毒素活性的表达依赖于独特的结构排列和构象。这样的分析进一步提供了对脂质A与人类靶细胞结合和激活所需的决定因素的洞察。目前的研究工作主要针对脂类A与宿主细胞相互作用的特异性、调节和生物医学后果的分子表征。
Lipopolysaccharides (LPS) constitute the O-antigens and endotoxins of Gram-negative bacteria. Whereas both the polysaccharide and lipid portion of LPS contribute to the pathogenic potential of this class of bacteria, it is the lipid component (lipid A) which determines die endotoxic properties of LPS. The primary structure of lipid A of various bacterial origin has been elucidated and Escherichia coli lipid A has been chemically synthesized. The biological analysis of synthetic lipid A partial structures proved that the expression of endotoxic activity depends on a unique structural arrangement and conformation. Such analyses have furthermore provided insight into the determinants required for lipid A binding to and activation of human target cells. Present research efforts aim at the molecular characterization of the specificity, modulation and biomedical consequences of the interaction of lipid A with host cells.