Effect of additives on gas-phase catalysis with immobilised Thermoanaerobacter species alcohol dehydrogenase (ADH T)
Effect of additives on gas-phase catalysis with immobilised Thermoanaerobacter species alcohol dehydrogenase (ADH T)
复制标题
添加剂对固定化热厌氧杆菌醇脱氢酶 (ADH T) 气相催化的影响
DOI：
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发表时间：
2006
影响因子：
5
通讯作者：
Jochen Büchs
中科院分区：
文献类型：
--
作者：
A. Trivedi;A. Spiess;T. Daussmann;Jochen Büchs
本文提出了一种用于气相反应的高效固定化乙醇脱氢酶制剂的制备策略。研究了缓冲液和蔗糖等添加剂对热厌氧菌乙醇脱氢酶的固定化效率（剩余活性和蛋白质负载）和气相反应效率（初始反应速率和半衰期）的影响。在固定的反应条件（温度和热力学活性）下，以异丙醇为共底物，在原位辅因子再生下将苯乙酮还原为1-苯乙醇作为模型反应。当酶用50 mM磷酸盐缓冲液（pH 7）固定时，酶在气相反应中的热稳定性得到了强烈增强，磷酸盐缓冲液中含有5倍于蛋白质量的蔗糖（以重量/重量为基础）。即使在非优化的反应条件下，也能达到200 g L−1 day−1的高产率。添加剂与酶和水的相互作用影响酶的固定化和气相效率。然而，即使知道了添加剂对固定效率的影响，也无法预测其对气相反应效率的影响。
This paper presents a strategy for preparing an efficient immobilised alcohol dehydrogenase preparation for a gas-phase reaction. The effects of additives such as buffers and sucrose on the immobilisation efficiency (residual activity and protein loading) and on the gas-phase reaction efficiency (initial reaction rate and half-life) of Thermoanaerobacter sp. alcohol dehydrogenase were studied. The reduction of acetophenone to 1-phenylethanol under in situ cofactor regeneration using isopropanol as co-substrate was used as a model reaction at fixed reaction conditions (temperature and thermodynamic activities). A strongly enhanced thermostability of the enzyme in the gas-phase reaction was achieved when the enzyme was immobilised with 50 mM phosphate buffer (pH 7) containing sucrose five times the protein amount (on weight/weight basis). This resulted in a remarkable productivity of 200 g L−1 day−1 even at non-optimised reaction conditions. The interaction of additives with the enzyme and water affects the immobilisation and gas-phase efficiencies of the enzyme. However, it was not possible to predict the effect of additives on the gas-phase reaction efficiency even after knowing their effect on the immobilisation efficiency.
影响因子：
17.3
作者：
Klibanov, AM
通讯作者：
Klibanov, AM