A Feasibility of Useful Cell-Based Therapy by Bone Regeneration with Deciduous Tooth Stem Cells, Dental Pulp Stem Cells, or Bone-Marrow-Derived Mesenchymal Stem Cells for Clinical Study Using Tissue Engineering Technology
A Feasibility of Useful Cell-Based Therapy by Bone Regeneration with Deciduous Tooth Stem Cells, Dental Pulp Stem Cells, or Bone-Marrow-Derived Mesenchymal Stem Cells for Clinical Study Using Tissue Engineering Technology
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DOI：
10.1089/ten.tea.2009.0732
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发表时间：
2010-06-01
影响因子：
4.1
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
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作者：
Yamada, Yoichi;Nakamura, Sayaka;Ueda, Minoru
本研究采用组织工程技术，将牙髓干细胞（DPSCs）、乳牙干细胞（DTSCs）和骨髓间充质干细胞（BMMSCs）分别用于羟基磷灰石涂层骨结合牙种植体的临床研究。在体外，人DPSC和DTSC表达STRO-1、CD 13、CD 29、CD 44、CD 73和成骨标志物基因，如碱性磷酸酶、Runx 2和骨钙素。在体内，使用以下移植材料植入制备的骨缺损模型：富血小板血浆（PRP）、PRP和犬BMMSC（cBMMSC）、PRP和犬DPSC（cDPSC）、PRP和幼犬DTSC（pDTSC）以及对照（仅缺损）。8周后，安装牙种植体，16周后，对切片进行组织学和组织计量学评价。cBMMSC/PRP、cDPSC/PRP和pDTSC/PRP组具有良好形成的成熟骨和新血管形成。组织学上，cBMMSCs/PRP、cDPSCs/PRP、pDTSCs/PRP组与对照组和PRP组之间的骨-植入物接触存在显著差异（p
This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue engineering technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone-implant contact was significantly different between the cBMMSCs/PRP, cDPSCs/PRP, pDTSCs/PRP groups, and the control and PRP groups (p