Free leptin is increased in normal pregnancy and further increased in preeclampsia
Free leptin is increased in normal pregnancy and further increased in preeclampsia
复制标题
DOI：
10.1053/meta.2000.7707
复制
发表时间：
2000-08-01
影响因子：
9.8
通讯作者：
Roberts, JM
中科院分区：
文献类型：
--
作者：
Teppa, RJ;Ness, RB;Roberts, JM
我们测定了子痫前期患者和配对的正常妊娠和未孕妇女的结合瘦素和游离瘦素水平，以确定瘦素的游离成分是否在妊娠期间增加，并在先兆子痫中进一步增加。18例正常孕妇和18例子痫前期患者孕前体重指数(BMI)匹配，18例未孕妇女BMI与妊娠组配对，取2毫升血清。样品用Sephadex G-100进行凝胶过滤。对各组分进行放射免疫分析(RIA)，并通过分析层析曲线下的面积来确定结合瘦素和游离瘦素的比例。孕妇血清总瘦素水平在正常妊娠组显著高于非妊娠组，子痫前期组进一步升高(分别为33.8±4.1vs15.2+/-1.8 ng/mL，P=0.002和48.1+/-5.6 ng/mL，P=0.02)。正常妊娠组血清游离瘦素水平明显高于未孕妇女(分别为25.9±4.1vs11.0+/-2.0 ng/mL，P=0.01)，而子痫前期组总瘦素水平仅在游离部分升高，显著高于正常妊娠组(分别为41.8±5.6vs25.9+/-4.1 ng/mL，P=0.01)。而正常妊娠组的瘦素结合分数显著高于子痫前期组和未孕组(7.9+/-0.56vs6.2+/-0.36和4.1+/-0.36 ng/mL，P=0.009和P=0.0001)。总之，游离瘦素浓度在正常妊娠时升高，在先兆子痫时进一步升高。这支持了这样的假设，即生物活性的瘦素在正常妊娠中升高，在先兆子痫妇女中增加更多。版权所有(C)2000，由W.B.Saunders公司提供。
We measured bound and free leptin levels in preeclamptic and matched normal pregnant and never-pregnant women to determine whether the free component of leptin is increased during pregnancy and further increased in preeclampsia. Two milliliters of serum was obtained from 18 normal and 18 preeclamptic patients matched by pre-pregnancy body mass index (BMI), and from 18 never-pregnant women matched by BMI with the pregnant groups. The sample was subjected to gel filtration using Sephadex G-100. Radioimmunoassay (RIA) was performed on all fractions, and the proportions of bound and free leptin were determined by analyzing the areas under the curve of the chromatographic profile. The total maternal serum leptin concentration was significantly higher in normal pregnancy compared with the nonpregnant state and was further increased in preeclampsia (33.8 +/- 4.1 v 15.2 +/- 1.8 ng/mL, P = .002, and 48.1 +/- 5.6 ng/mL, P = .02, respectively). Free leptin was increased in normal pregnant compared with never-pregnant women (25.9 +/- 4.1 v 11.0 +/- 2.0 ng/mL, respectively, P = .01), while the increase of total leptin in preeclampsia was exclusively in the free fraction that was significantly higher versus the normal pregnant group (41.8 +/- 5.6 v 25.9 +/- 4.1 ng/mL, respectively, P = .01). The bound leptin fraction, by contrast, was significantly increased in the normal pregnant group compared with the preeclamptic group and the never-pregnant group (7.9 +/- 0.56 v 6.2 +/- 0.36 and 4.1 +/- 0.36 ng/mL, respectively, P = .009 and P = < .0001). In conclusion, the free leptin concentration increases in normal pregnancy and is further increased in preeclampsia. This supports the hypothesis that biologically active leptin is elevated in normal pregnancy and is increased more in women with preeclampsia. Copyright (C) 2000 by W.B. Saunders Company.